Impaired coordination of nucleophile and increased hydrophobicity in the +1 subsite shift levansucrase activity towards transfructosylation

Bacterial levansucrases produce β(2,6)-linked levan-type polysaccharides using sucrose or sucrose analogs as donor/acceptor substrates. However, the dominant reaction of Bacillus megaterium levansucrase (Bm-LS) is hydrolysis. Single domain levansucrases from Gram-positive bacteria display a wide sub...

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Detalles Bibliográficos
Autores principales: Ortiz-Soto, Maria Elena, Possiel, Christian, Görl, Julian, Vogel, Andreas, Schmiedel, Ramona, Seibel, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Original articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881714/
https://www.ncbi.nlm.nih.gov/pubmed/28575294
http://dx.doi.org/10.1093/glycob/cwx050

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881714/
https://www.ncbi.nlm.nih.gov/pubmed/28575294
http://dx.doi.org/10.1093/glycob/cwx050

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