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Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats

Microglia, which comprise a sensor for pathological events in the central nervous system, may be triggered by nerve injury and transformed from a quiescent state into an activated state; ionised calcium binding adaptor molecule 1 (Iba1) is a sensitive marker associated with activated microglia. Accu...

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Autores principales: Tu, Wen-Zhan, Li, Si-Si, Jiang, Xia, Qian, Xin-Ru, Yang, Guan-Hu, Gu, Peng-Peng, Lu, Bin, Jiang, Song-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881805/
https://www.ncbi.nlm.nih.gov/pubmed/29568892
http://dx.doi.org/10.3892/ijmm.2018.3563
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author Tu, Wen-Zhan
Li, Si-Si
Jiang, Xia
Qian, Xin-Ru
Yang, Guan-Hu
Gu, Peng-Peng
Lu, Bin
Jiang, Song-He
author_facet Tu, Wen-Zhan
Li, Si-Si
Jiang, Xia
Qian, Xin-Ru
Yang, Guan-Hu
Gu, Peng-Peng
Lu, Bin
Jiang, Song-He
author_sort Tu, Wen-Zhan
collection PubMed
description Microglia, which comprise a sensor for pathological events in the central nervous system, may be triggered by nerve injury and transformed from a quiescent state into an activated state; ionised calcium binding adaptor molecule 1 (Iba1) is a sensitive marker associated with activated microglia. Accumulated evidence suggests that spinal activated microglia and the brain-derived neurotrophic factor (BDNF)-tyrosine kinase receptor B (TrkB) signalling pathway play major roles in the production and development of neuropathic pain. Electro-acupuncture (EA) has a positive effect on relieving chronic neuropathic pain; however, the underlying mechanisms remain unclear. To determine the significance of EA in the treatment of neuropathic pain mediated by activated microglia and the BDNF-TrkB signalling pathway in the spinal cord, the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) values were recorded to assess hyperalgesia and allodynia. In addition, the amount of activated microglia and BDNF were assessed via immunofluorescence. Iba1, BDNF and TrkB mRNA expression levels were examined using qPCR; the protein levels of BDNF, p-TrkB and TrkB in the spinal cord were analysed via western blotting. The present study demonstrated that EA treatment increased the MWT and TWL values. EA significantly inhibited the proportion of activated microglia and BDNF expression in the spinal cord after chronic constrictive injury (CCI). Furthermore, EA decreased the expression of BDNF and TrkB at both the mRNA and protein levels in the spinal cord of CCI rats. These findings suggest that the analgesic effect of EA may be achieved by inhibiting the activation of spinal microglia and subsequently blocking the BDNF-TrkB signalling pathway.
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spelling pubmed-58818052018-04-12 Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats Tu, Wen-Zhan Li, Si-Si Jiang, Xia Qian, Xin-Ru Yang, Guan-Hu Gu, Peng-Peng Lu, Bin Jiang, Song-He Int J Mol Med Articles Microglia, which comprise a sensor for pathological events in the central nervous system, may be triggered by nerve injury and transformed from a quiescent state into an activated state; ionised calcium binding adaptor molecule 1 (Iba1) is a sensitive marker associated with activated microglia. Accumulated evidence suggests that spinal activated microglia and the brain-derived neurotrophic factor (BDNF)-tyrosine kinase receptor B (TrkB) signalling pathway play major roles in the production and development of neuropathic pain. Electro-acupuncture (EA) has a positive effect on relieving chronic neuropathic pain; however, the underlying mechanisms remain unclear. To determine the significance of EA in the treatment of neuropathic pain mediated by activated microglia and the BDNF-TrkB signalling pathway in the spinal cord, the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) values were recorded to assess hyperalgesia and allodynia. In addition, the amount of activated microglia and BDNF were assessed via immunofluorescence. Iba1, BDNF and TrkB mRNA expression levels were examined using qPCR; the protein levels of BDNF, p-TrkB and TrkB in the spinal cord were analysed via western blotting. The present study demonstrated that EA treatment increased the MWT and TWL values. EA significantly inhibited the proportion of activated microglia and BDNF expression in the spinal cord after chronic constrictive injury (CCI). Furthermore, EA decreased the expression of BDNF and TrkB at both the mRNA and protein levels in the spinal cord of CCI rats. These findings suggest that the analgesic effect of EA may be achieved by inhibiting the activation of spinal microglia and subsequently blocking the BDNF-TrkB signalling pathway. D.A. Spandidos 2018-06 2018-03-13 /pmc/articles/PMC5881805/ /pubmed/29568892 http://dx.doi.org/10.3892/ijmm.2018.3563 Text en Copyright: © Tu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tu, Wen-Zhan
Li, Si-Si
Jiang, Xia
Qian, Xin-Ru
Yang, Guan-Hu
Gu, Peng-Peng
Lu, Bin
Jiang, Song-He
Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats
title Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats
title_full Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats
title_fullStr Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats
title_full_unstemmed Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats
title_short Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats
title_sort effect of electro-acupuncture on the bdnf-trkb pathway in the spinal cord of cci rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881805/
https://www.ncbi.nlm.nih.gov/pubmed/29568892
http://dx.doi.org/10.3892/ijmm.2018.3563
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