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Enhanced labeling density and whole-cell 3D dSTORM imaging by repetitive labeling of target proteins
With continuing advances in the resolving power of super-resolution microscopy, the inefficient labeling of proteins with suitable fluorophores becomes a limiting factor. For example, the low labeling density achieved with antibodies or small molecule tags limits attempts to reveal local protein nan...
Autores principales: | Venkataramani, Varun, Kardorff, Markus, Herrmannsdörfer, Frank, Wieneke, Ralph, Klein, Alina, Tampé, Robert, Heilemann, Mike, Kuner, Thomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882651/ https://www.ncbi.nlm.nih.gov/pubmed/29615726 http://dx.doi.org/10.1038/s41598-018-23818-0 |
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