Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging

This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs...

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Autores principales: Vijaya Bharathi, M., Maiti, Santanu, Sarkar, Bidisha, Ghosh, Kaustab, Paira, Priyankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society Publishing 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882692/
https://www.ncbi.nlm.nih.gov/pubmed/29657768
http://dx.doi.org/10.1098/rsos.171614
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author Vijaya Bharathi, M.
Maiti, Santanu
Sarkar, Bidisha
Ghosh, Kaustab
Paira, Priyankar
author_facet Vijaya Bharathi, M.
Maiti, Santanu
Sarkar, Bidisha
Ghosh, Kaustab
Paira, Priyankar
author_sort Vijaya Bharathi, M.
collection PubMed
description This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs were further modified with streptavidin and then bound to biotin because of its high conjugation efficiency. Labelling and bio-imaging of cells with these bio-conjugated QDs were evaluated. The bright red fluorescence from these types of QDs in HeLa cells makes these materials suitable for deep tissue imaging.
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spelling pubmed-58826922018-04-13 Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging Vijaya Bharathi, M. Maiti, Santanu Sarkar, Bidisha Ghosh, Kaustab Paira, Priyankar R Soc Open Sci Chemistry This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs were further modified with streptavidin and then bound to biotin because of its high conjugation efficiency. Labelling and bio-imaging of cells with these bio-conjugated QDs were evaluated. The bright red fluorescence from these types of QDs in HeLa cells makes these materials suitable for deep tissue imaging. The Royal Society Publishing 2018-03-14 /pmc/articles/PMC5882692/ /pubmed/29657768 http://dx.doi.org/10.1098/rsos.171614 Text en © 2018 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Chemistry
Vijaya Bharathi, M.
Maiti, Santanu
Sarkar, Bidisha
Ghosh, Kaustab
Paira, Priyankar
Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
title Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
title_full Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
title_fullStr Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
title_full_unstemmed Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
title_short Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
title_sort water-mediated green synthesis of pbs quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882692/
https://www.ncbi.nlm.nih.gov/pubmed/29657768
http://dx.doi.org/10.1098/rsos.171614
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