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Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.)
Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification of a common midwife toad virus (CMTV) associated with massive die-offs in water frogs (Pelophylax spp.) in the Netherlands has increased awareness for emerging viruses in amphibians in the country. Comple...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882854/ https://www.ncbi.nlm.nih.gov/pubmed/29615625 http://dx.doi.org/10.1038/s41426-018-0058-5 |
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author | Saucedo, Bernardo Hughes, Joseph Spitzen-van der Sluijs, Annemarieke Kruithof, Natasja Schills, Marc Rijks, Jolianne M. Jacinto-Maldonado, Mónica Suarez, Nicolás Haenen, Olga L. M. Voorbergen-Laarman, Michal van den Broek, Jan Gilbert, Maarten Gröne, Andrea van Beurden, Steven J. Verheije, M. Hélène |
author_facet | Saucedo, Bernardo Hughes, Joseph Spitzen-van der Sluijs, Annemarieke Kruithof, Natasja Schills, Marc Rijks, Jolianne M. Jacinto-Maldonado, Mónica Suarez, Nicolás Haenen, Olga L. M. Voorbergen-Laarman, Michal van den Broek, Jan Gilbert, Maarten Gröne, Andrea van Beurden, Steven J. Verheije, M. Hélène |
author_sort | Saucedo, Bernardo |
collection | PubMed |
description | Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification of a common midwife toad virus (CMTV) associated with massive die-offs in water frogs (Pelophylax spp.) in the Netherlands has increased awareness for emerging viruses in amphibians in the country. Complete genome sequencing of 13 ranavirus isolates collected from ten different sites in the period 2011–2016 revealed three CMTV groups present in distinct geographical areas in the Netherlands. Phylogenetic analysis showed that emerging viruses from the northern part of the Netherlands belonged to CMTV-NL group I. Group II and III viruses were derived from the animals located in the center-east and south of the country, and shared a more recent common ancestor to CMTV-amphibian associated ranaviruses reported in China, Italy, Denmark, and Switzerland. Field monitoring revealed differences in water frog host abundance at sites where distinct ranavirus groups occur; with ranavirus-associated deaths, host counts decreasing progressively, and few juveniles found in the north where CMTV-NL group I occurs but not in the south with CMTV-NL group III. Investigation of tandem repeats of coding genes gave no conclusive information about phylo-geographical clustering, while genetic analysis of the genomes revealed truncations in 17 genes across CMTV-NL groups II and III compared to group I. Further studies are needed to elucidate the contribution of these genes as well as environmental variables to explain the observed differences in host abundance. |
format | Online Article Text |
id | pubmed-5882854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58828542018-04-06 Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) Saucedo, Bernardo Hughes, Joseph Spitzen-van der Sluijs, Annemarieke Kruithof, Natasja Schills, Marc Rijks, Jolianne M. Jacinto-Maldonado, Mónica Suarez, Nicolás Haenen, Olga L. M. Voorbergen-Laarman, Michal van den Broek, Jan Gilbert, Maarten Gröne, Andrea van Beurden, Steven J. Verheije, M. Hélène Emerg Microbes Infect Article Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification of a common midwife toad virus (CMTV) associated with massive die-offs in water frogs (Pelophylax spp.) in the Netherlands has increased awareness for emerging viruses in amphibians in the country. Complete genome sequencing of 13 ranavirus isolates collected from ten different sites in the period 2011–2016 revealed three CMTV groups present in distinct geographical areas in the Netherlands. Phylogenetic analysis showed that emerging viruses from the northern part of the Netherlands belonged to CMTV-NL group I. Group II and III viruses were derived from the animals located in the center-east and south of the country, and shared a more recent common ancestor to CMTV-amphibian associated ranaviruses reported in China, Italy, Denmark, and Switzerland. Field monitoring revealed differences in water frog host abundance at sites where distinct ranavirus groups occur; with ranavirus-associated deaths, host counts decreasing progressively, and few juveniles found in the north where CMTV-NL group I occurs but not in the south with CMTV-NL group III. Investigation of tandem repeats of coding genes gave no conclusive information about phylo-geographical clustering, while genetic analysis of the genomes revealed truncations in 17 genes across CMTV-NL groups II and III compared to group I. Further studies are needed to elucidate the contribution of these genes as well as environmental variables to explain the observed differences in host abundance. Nature Publishing Group UK 2018-04-04 /pmc/articles/PMC5882854/ /pubmed/29615625 http://dx.doi.org/10.1038/s41426-018-0058-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Saucedo, Bernardo Hughes, Joseph Spitzen-van der Sluijs, Annemarieke Kruithof, Natasja Schills, Marc Rijks, Jolianne M. Jacinto-Maldonado, Mónica Suarez, Nicolás Haenen, Olga L. M. Voorbergen-Laarman, Michal van den Broek, Jan Gilbert, Maarten Gröne, Andrea van Beurden, Steven J. Verheije, M. Hélène Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) |
title | Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) |
title_full | Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) |
title_fullStr | Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) |
title_full_unstemmed | Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) |
title_short | Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.) |
title_sort | ranavirus genotypes in the netherlands and their potential association with virulence in water frogs (pelophylax spp.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882854/ https://www.ncbi.nlm.nih.gov/pubmed/29615625 http://dx.doi.org/10.1038/s41426-018-0058-5 |
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