A porcine ex vivo model of pigmentary glaucoma

Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo mod...

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Autores principales: Dang, Yalong, Waxman, Susannah, Wang, Chao, Loewen, Ralitsa T., Sun, Ming, Loewen, Nils A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882895/
https://www.ncbi.nlm.nih.gov/pubmed/29615741
http://dx.doi.org/10.1038/s41598-018-23861-x
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author Dang, Yalong
Waxman, Susannah
Wang, Chao
Loewen, Ralitsa T.
Sun, Ming
Loewen, Nils A.
author_facet Dang, Yalong
Waxman, Susannah
Wang, Chao
Loewen, Ralitsa T.
Sun, Ming
Loewen, Nils A.
author_sort Dang, Yalong
collection PubMed
description Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo model that recreates several features of pigmentary glaucoma, including intraocular hypertension, accumulation of pigment in the trabecular meshwork, and declining phagocytosis. We found that trabecular meshwork cells regulate outflow, form actin stress fibers, and have a decreased phagocytic activity. Gene expression microarrays and a pathway analysis of TM monolayers as well as ex vivo anterior segment perfusion cultures indicated that RhoA plays a central role in regulating the cytoskeleton, motility, and phagocytosis in the trabecular meshwork, providing new insights and targets to investigate in pigmentary glaucoma.
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spelling pubmed-58828952018-04-09 A porcine ex vivo model of pigmentary glaucoma Dang, Yalong Waxman, Susannah Wang, Chao Loewen, Ralitsa T. Sun, Ming Loewen, Nils A. Sci Rep Article Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo model that recreates several features of pigmentary glaucoma, including intraocular hypertension, accumulation of pigment in the trabecular meshwork, and declining phagocytosis. We found that trabecular meshwork cells regulate outflow, form actin stress fibers, and have a decreased phagocytic activity. Gene expression microarrays and a pathway analysis of TM monolayers as well as ex vivo anterior segment perfusion cultures indicated that RhoA plays a central role in regulating the cytoskeleton, motility, and phagocytosis in the trabecular meshwork, providing new insights and targets to investigate in pigmentary glaucoma. Nature Publishing Group UK 2018-04-03 /pmc/articles/PMC5882895/ /pubmed/29615741 http://dx.doi.org/10.1038/s41598-018-23861-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dang, Yalong
Waxman, Susannah
Wang, Chao
Loewen, Ralitsa T.
Sun, Ming
Loewen, Nils A.
A porcine ex vivo model of pigmentary glaucoma
title A porcine ex vivo model of pigmentary glaucoma
title_full A porcine ex vivo model of pigmentary glaucoma
title_fullStr A porcine ex vivo model of pigmentary glaucoma
title_full_unstemmed A porcine ex vivo model of pigmentary glaucoma
title_short A porcine ex vivo model of pigmentary glaucoma
title_sort porcine ex vivo model of pigmentary glaucoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882895/
https://www.ncbi.nlm.nih.gov/pubmed/29615741
http://dx.doi.org/10.1038/s41598-018-23861-x
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