Cargando…

Silent infection of human dendritic cells by African and Asian strains of Zika virus

While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonate...

Descripción completa

Detalles Bibliográficos
Autores principales: Vielle, Nathalie J., Zumkehr, Beatrice, García-Nicolás, Obdulio, Blank, Fabian, Stojanov, Miloš, Musso, Didier, Baud, David, Summerfield, Artur, Alves, Marco P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882923/
https://www.ncbi.nlm.nih.gov/pubmed/29615676
http://dx.doi.org/10.1038/s41598-018-23734-3
_version_ 1783311553433436160
author Vielle, Nathalie J.
Zumkehr, Beatrice
García-Nicolás, Obdulio
Blank, Fabian
Stojanov, Miloš
Musso, Didier
Baud, David
Summerfield, Artur
Alves, Marco P.
author_facet Vielle, Nathalie J.
Zumkehr, Beatrice
García-Nicolás, Obdulio
Blank, Fabian
Stojanov, Miloš
Musso, Didier
Baud, David
Summerfield, Artur
Alves, Marco P.
author_sort Vielle, Nathalie J.
collection PubMed
description While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made.
format Online
Article
Text
id pubmed-5882923
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-58829232018-04-09 Silent infection of human dendritic cells by African and Asian strains of Zika virus Vielle, Nathalie J. Zumkehr, Beatrice García-Nicolás, Obdulio Blank, Fabian Stojanov, Miloš Musso, Didier Baud, David Summerfield, Artur Alves, Marco P. Sci Rep Article While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made. Nature Publishing Group UK 2018-04-03 /pmc/articles/PMC5882923/ /pubmed/29615676 http://dx.doi.org/10.1038/s41598-018-23734-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Vielle, Nathalie J.
Zumkehr, Beatrice
García-Nicolás, Obdulio
Blank, Fabian
Stojanov, Miloš
Musso, Didier
Baud, David
Summerfield, Artur
Alves, Marco P.
Silent infection of human dendritic cells by African and Asian strains of Zika virus
title Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_full Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_fullStr Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_full_unstemmed Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_short Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_sort silent infection of human dendritic cells by african and asian strains of zika virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882923/
https://www.ncbi.nlm.nih.gov/pubmed/29615676
http://dx.doi.org/10.1038/s41598-018-23734-3
work_keys_str_mv AT viellenathaliej silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT zumkehrbeatrice silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT garcianicolasobdulio silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT blankfabian silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT stojanovmilos silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT mussodidier silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT bauddavid silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT summerfieldartur silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
AT alvesmarcop silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus