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Silent infection of human dendritic cells by African and Asian strains of Zika virus
While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonate...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882923/ https://www.ncbi.nlm.nih.gov/pubmed/29615676 http://dx.doi.org/10.1038/s41598-018-23734-3 |
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author | Vielle, Nathalie J. Zumkehr, Beatrice García-Nicolás, Obdulio Blank, Fabian Stojanov, Miloš Musso, Didier Baud, David Summerfield, Artur Alves, Marco P. |
author_facet | Vielle, Nathalie J. Zumkehr, Beatrice García-Nicolás, Obdulio Blank, Fabian Stojanov, Miloš Musso, Didier Baud, David Summerfield, Artur Alves, Marco P. |
author_sort | Vielle, Nathalie J. |
collection | PubMed |
description | While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made. |
format | Online Article Text |
id | pubmed-5882923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58829232018-04-09 Silent infection of human dendritic cells by African and Asian strains of Zika virus Vielle, Nathalie J. Zumkehr, Beatrice García-Nicolás, Obdulio Blank, Fabian Stojanov, Miloš Musso, Didier Baud, David Summerfield, Artur Alves, Marco P. Sci Rep Article While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made. Nature Publishing Group UK 2018-04-03 /pmc/articles/PMC5882923/ /pubmed/29615676 http://dx.doi.org/10.1038/s41598-018-23734-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Vielle, Nathalie J. Zumkehr, Beatrice García-Nicolás, Obdulio Blank, Fabian Stojanov, Miloš Musso, Didier Baud, David Summerfield, Artur Alves, Marco P. Silent infection of human dendritic cells by African and Asian strains of Zika virus |
title | Silent infection of human dendritic cells by African and Asian strains of Zika virus |
title_full | Silent infection of human dendritic cells by African and Asian strains of Zika virus |
title_fullStr | Silent infection of human dendritic cells by African and Asian strains of Zika virus |
title_full_unstemmed | Silent infection of human dendritic cells by African and Asian strains of Zika virus |
title_short | Silent infection of human dendritic cells by African and Asian strains of Zika virus |
title_sort | silent infection of human dendritic cells by african and asian strains of zika virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882923/ https://www.ncbi.nlm.nih.gov/pubmed/29615676 http://dx.doi.org/10.1038/s41598-018-23734-3 |
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