High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs

The cultivated peanut, A. hypogaea L., is an important oil and food crop globally.High-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. In this context, a recombinant inbred line (RIL) of 146 lines was developed by crossing Huayu28 and P76...

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Autores principales: Hu, X. H., Zhang, S. Z., Miao, H. R., Cui, F. G., Shen, Y., Yang, W. Q., Xu, T. T., Chen, N., Chi, X. Y., Zhang, Z. M., Chen, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883025/
https://www.ncbi.nlm.nih.gov/pubmed/29615772
http://dx.doi.org/10.1038/s41598-018-23873-7
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author Hu, X. H.
Zhang, S. Z.
Miao, H. R.
Cui, F. G.
Shen, Y.
Yang, W. Q.
Xu, T. T.
Chen, N.
Chi, X. Y.
Zhang, Z. M.
Chen, J.
author_facet Hu, X. H.
Zhang, S. Z.
Miao, H. R.
Cui, F. G.
Shen, Y.
Yang, W. Q.
Xu, T. T.
Chen, N.
Chi, X. Y.
Zhang, Z. M.
Chen, J.
author_sort Hu, X. H.
collection PubMed
description The cultivated peanut, A. hypogaea L., is an important oil and food crop globally.High-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. In this context, a recombinant inbred line (RIL) of 146 lines was developed by crossing Huayu28 and P76. We developed 433,679 high-quality SLAFs, of which 29,075 were polymorphic. 4,817 SLAFs were encoded and grouped into different segregation patterns. A high-resolution genetic map containing 2,334 markers (68 SSRs and 2,266 SNPs) on 20 linkage groups (LGs) spanning 2586.37 cM was constructed for peanut. The average distance between adjacent markers was 2.25 cM. Based on phenotyping in seven environments, QTLs for oleic acid (C18:1), linoleic acid (C18:2) and the ratio of oleic acid to linoleic acid (O/L) were identified and positioned on linkage groups A03, A04, A09, B09 and B10. Marker2575339 and Marker2379598 in B09 were associated with C18:1, C18:2 and O/L in seven environments, Marker4391589 and Marker4463600 in A09 were associated with C18:1, C18:2 and O/L in six environments. This map exhibits high resolution and accuracy, which will facilitate QTL discovery for essential agronomic traits in peanut.
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spelling pubmed-58830252018-04-09 High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs Hu, X. H. Zhang, S. Z. Miao, H. R. Cui, F. G. Shen, Y. Yang, W. Q. Xu, T. T. Chen, N. Chi, X. Y. Zhang, Z. M. Chen, J. Sci Rep Article The cultivated peanut, A. hypogaea L., is an important oil and food crop globally.High-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. In this context, a recombinant inbred line (RIL) of 146 lines was developed by crossing Huayu28 and P76. We developed 433,679 high-quality SLAFs, of which 29,075 were polymorphic. 4,817 SLAFs were encoded and grouped into different segregation patterns. A high-resolution genetic map containing 2,334 markers (68 SSRs and 2,266 SNPs) on 20 linkage groups (LGs) spanning 2586.37 cM was constructed for peanut. The average distance between adjacent markers was 2.25 cM. Based on phenotyping in seven environments, QTLs for oleic acid (C18:1), linoleic acid (C18:2) and the ratio of oleic acid to linoleic acid (O/L) were identified and positioned on linkage groups A03, A04, A09, B09 and B10. Marker2575339 and Marker2379598 in B09 were associated with C18:1, C18:2 and O/L in seven environments, Marker4391589 and Marker4463600 in A09 were associated with C18:1, C18:2 and O/L in six environments. This map exhibits high resolution and accuracy, which will facilitate QTL discovery for essential agronomic traits in peanut. Nature Publishing Group UK 2018-04-03 /pmc/articles/PMC5883025/ /pubmed/29615772 http://dx.doi.org/10.1038/s41598-018-23873-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hu, X. H.
Zhang, S. Z.
Miao, H. R.
Cui, F. G.
Shen, Y.
Yang, W. Q.
Xu, T. T.
Chen, N.
Chi, X. Y.
Zhang, Z. M.
Chen, J.
High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs
title High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs
title_full High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs
title_fullStr High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs
title_full_unstemmed High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs
title_short High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs
title_sort high-density genetic map construction and identification of qtls controlling oleic and linoleic acid in peanut using slaf-seq and ssrs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883025/
https://www.ncbi.nlm.nih.gov/pubmed/29615772
http://dx.doi.org/10.1038/s41598-018-23873-7
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