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Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure
Extraction of cell-free DNA (cfDNA), which exists at an extremely low concentration in plasma, is a critical process for either targeted-sensing or massive sequencing of DNAs. However, such small amount of DNA cannot be fully obtained without high-speed centrifugation (<20,000 g). Here, we develo...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883035/ https://www.ncbi.nlm.nih.gov/pubmed/29615736 http://dx.doi.org/10.1038/s41598-018-23766-9 |