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Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells

Muscle atrophy is one of the main characteristics of human ageing and physical inactivity, with resulting adverse health outcomes. To date, there are still no efficient therapeutic strategies for its prevention and/or treatment. However, during hibernation, bears exhibit a unique ability for preserv...

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Autores principales: Chanon, Stéphanie, Chazarin, Blandine, Toubhans, Benoit, Durand, Christine, Chery, Isabelle, Robert, Maud, Vieille-Marchiset, Aurélie, Swenson, Jon E., Zedrosser, Andreas, Evans, Alina L., Brunberg, Sven, Arnemo, Jon M., Gauquelin-Koch, Guillemette, Storey, Kenneth B., Simon, Chantal, Blanc, Stéphane, Bertile, Fabrice, Lefai, Etienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883044/
https://www.ncbi.nlm.nih.gov/pubmed/29615761
http://dx.doi.org/10.1038/s41598-018-23891-5
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author Chanon, Stéphanie
Chazarin, Blandine
Toubhans, Benoit
Durand, Christine
Chery, Isabelle
Robert, Maud
Vieille-Marchiset, Aurélie
Swenson, Jon E.
Zedrosser, Andreas
Evans, Alina L.
Brunberg, Sven
Arnemo, Jon M.
Gauquelin-Koch, Guillemette
Storey, Kenneth B.
Simon, Chantal
Blanc, Stéphane
Bertile, Fabrice
Lefai, Etienne
author_facet Chanon, Stéphanie
Chazarin, Blandine
Toubhans, Benoit
Durand, Christine
Chery, Isabelle
Robert, Maud
Vieille-Marchiset, Aurélie
Swenson, Jon E.
Zedrosser, Andreas
Evans, Alina L.
Brunberg, Sven
Arnemo, Jon M.
Gauquelin-Koch, Guillemette
Storey, Kenneth B.
Simon, Chantal
Blanc, Stéphane
Bertile, Fabrice
Lefai, Etienne
author_sort Chanon, Stéphanie
collection PubMed
description Muscle atrophy is one of the main characteristics of human ageing and physical inactivity, with resulting adverse health outcomes. To date, there are still no efficient therapeutic strategies for its prevention and/or treatment. However, during hibernation, bears exhibit a unique ability for preserving muscle in conditions where muscle atrophy would be expected in humans. Therefore, our objective was to determine whether there are components of bear serum which can control protein balance in human muscles. In this study, we exposed cultured human differentiated muscle cells to bear serum collected during winter and summer periods, and measured the impact on cell protein content and turnover. In addition, we explored the signalling pathways that control rates of protein synthesis and degradation. We show that the protein turnover of human myotubes is reduced when incubated with winter bear serum, with a dramatic inhibition of proteolysis involving both proteasomal and lysosomal systems, and resulting in an increase in muscle cell protein content. By modulating intracellular signalling pathways and inducing a protein sparing phenotype in human muscle cells, winter bear serum therefore holds potential for developing new tools to fight human muscle atrophy and related metabolic disorders.
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spelling pubmed-58830442018-04-09 Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells Chanon, Stéphanie Chazarin, Blandine Toubhans, Benoit Durand, Christine Chery, Isabelle Robert, Maud Vieille-Marchiset, Aurélie Swenson, Jon E. Zedrosser, Andreas Evans, Alina L. Brunberg, Sven Arnemo, Jon M. Gauquelin-Koch, Guillemette Storey, Kenneth B. Simon, Chantal Blanc, Stéphane Bertile, Fabrice Lefai, Etienne Sci Rep Article Muscle atrophy is one of the main characteristics of human ageing and physical inactivity, with resulting adverse health outcomes. To date, there are still no efficient therapeutic strategies for its prevention and/or treatment. However, during hibernation, bears exhibit a unique ability for preserving muscle in conditions where muscle atrophy would be expected in humans. Therefore, our objective was to determine whether there are components of bear serum which can control protein balance in human muscles. In this study, we exposed cultured human differentiated muscle cells to bear serum collected during winter and summer periods, and measured the impact on cell protein content and turnover. In addition, we explored the signalling pathways that control rates of protein synthesis and degradation. We show that the protein turnover of human myotubes is reduced when incubated with winter bear serum, with a dramatic inhibition of proteolysis involving both proteasomal and lysosomal systems, and resulting in an increase in muscle cell protein content. By modulating intracellular signalling pathways and inducing a protein sparing phenotype in human muscle cells, winter bear serum therefore holds potential for developing new tools to fight human muscle atrophy and related metabolic disorders. Nature Publishing Group UK 2018-04-03 /pmc/articles/PMC5883044/ /pubmed/29615761 http://dx.doi.org/10.1038/s41598-018-23891-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chanon, Stéphanie
Chazarin, Blandine
Toubhans, Benoit
Durand, Christine
Chery, Isabelle
Robert, Maud
Vieille-Marchiset, Aurélie
Swenson, Jon E.
Zedrosser, Andreas
Evans, Alina L.
Brunberg, Sven
Arnemo, Jon M.
Gauquelin-Koch, Guillemette
Storey, Kenneth B.
Simon, Chantal
Blanc, Stéphane
Bertile, Fabrice
Lefai, Etienne
Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
title Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
title_full Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
title_fullStr Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
title_full_unstemmed Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
title_short Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
title_sort proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883044/
https://www.ncbi.nlm.nih.gov/pubmed/29615761
http://dx.doi.org/10.1038/s41598-018-23891-5
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