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Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium

BACKGROUND: In the context of malaria elimination/eradication, drugs that are effective against the different developmental stages of the parasite are highly desirable. The oldest synthetic anti-malarial drug, the thiazine dye methylene blue (MB), is known for its activity against Plasmodium blood s...

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Autores principales: Bosson-Vanga, Henriette, Franetich, Jean-François, Soulard, Valérie, Sossau, Daniel, Tefit, Maurel, Kane, Bocar, Vaillant, Jean-Christophe, Borrmann, Steffen, Müller, Olaf, Dereuddre-Bosquet, Nathalie, Le Grand, Roger, Silvie, Olivier, Mazier, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883292/
https://www.ncbi.nlm.nih.gov/pubmed/29615050
http://dx.doi.org/10.1186/s12936-018-2300-y
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author Bosson-Vanga, Henriette
Franetich, Jean-François
Soulard, Valérie
Sossau, Daniel
Tefit, Maurel
Kane, Bocar
Vaillant, Jean-Christophe
Borrmann, Steffen
Müller, Olaf
Dereuddre-Bosquet, Nathalie
Le Grand, Roger
Silvie, Olivier
Mazier, Dominique
author_facet Bosson-Vanga, Henriette
Franetich, Jean-François
Soulard, Valérie
Sossau, Daniel
Tefit, Maurel
Kane, Bocar
Vaillant, Jean-Christophe
Borrmann, Steffen
Müller, Olaf
Dereuddre-Bosquet, Nathalie
Le Grand, Roger
Silvie, Olivier
Mazier, Dominique
author_sort Bosson-Vanga, Henriette
collection PubMed
description BACKGROUND: In the context of malaria elimination/eradication, drugs that are effective against the different developmental stages of the parasite are highly desirable. The oldest synthetic anti-malarial drug, the thiazine dye methylene blue (MB), is known for its activity against Plasmodium blood stages, including gametocytes. The aim of the present study was to investigate a possible effect of MB against malaria parasite liver stages. METHODS: MB activity was investigated using both in vitro and in vivo models. In vitro assays consisted of testing MB activity on Plasmodium falciparum, Plasmodium cynomolgi and Plasmodium yoelii parasites in human, simian or murine primary hepatocytes, respectively. MB in vivo activity was evaluated using intravital imaging in BALB/c mice infected with a transgenic bioluminescent P. yoelii parasite line. The transmission-blocking activity of MB was also addressed using mosquitoes fed on MB-treated mice. RESULTS: MB shows no activity on Plasmodium liver stages, including hypnozoites, in vitro in primary hepatocytes. In BALB/c mice, MB has moderate effect on P. yoelii hepatic development but is highly effective against blood stage growth. MB is active against gametocytes and abrogates parasite transmission from mice to mosquitoes. CONCLUSION: While confirming activity of MB against both sexual and asexual blood stages, the results indicate that MB has only little activity on the development of the hepatic stages of malaria parasites.
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spelling pubmed-58832922018-04-10 Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium Bosson-Vanga, Henriette Franetich, Jean-François Soulard, Valérie Sossau, Daniel Tefit, Maurel Kane, Bocar Vaillant, Jean-Christophe Borrmann, Steffen Müller, Olaf Dereuddre-Bosquet, Nathalie Le Grand, Roger Silvie, Olivier Mazier, Dominique Malar J Research BACKGROUND: In the context of malaria elimination/eradication, drugs that are effective against the different developmental stages of the parasite are highly desirable. The oldest synthetic anti-malarial drug, the thiazine dye methylene blue (MB), is known for its activity against Plasmodium blood stages, including gametocytes. The aim of the present study was to investigate a possible effect of MB against malaria parasite liver stages. METHODS: MB activity was investigated using both in vitro and in vivo models. In vitro assays consisted of testing MB activity on Plasmodium falciparum, Plasmodium cynomolgi and Plasmodium yoelii parasites in human, simian or murine primary hepatocytes, respectively. MB in vivo activity was evaluated using intravital imaging in BALB/c mice infected with a transgenic bioluminescent P. yoelii parasite line. The transmission-blocking activity of MB was also addressed using mosquitoes fed on MB-treated mice. RESULTS: MB shows no activity on Plasmodium liver stages, including hypnozoites, in vitro in primary hepatocytes. In BALB/c mice, MB has moderate effect on P. yoelii hepatic development but is highly effective against blood stage growth. MB is active against gametocytes and abrogates parasite transmission from mice to mosquitoes. CONCLUSION: While confirming activity of MB against both sexual and asexual blood stages, the results indicate that MB has only little activity on the development of the hepatic stages of malaria parasites. BioMed Central 2018-04-03 /pmc/articles/PMC5883292/ /pubmed/29615050 http://dx.doi.org/10.1186/s12936-018-2300-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Bosson-Vanga, Henriette
Franetich, Jean-François
Soulard, Valérie
Sossau, Daniel
Tefit, Maurel
Kane, Bocar
Vaillant, Jean-Christophe
Borrmann, Steffen
Müller, Olaf
Dereuddre-Bosquet, Nathalie
Le Grand, Roger
Silvie, Olivier
Mazier, Dominique
Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
title Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
title_full Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
title_fullStr Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
title_full_unstemmed Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
title_short Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
title_sort differential activity of methylene blue against erythrocytic and hepatic stages of plasmodium
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883292/
https://www.ncbi.nlm.nih.gov/pubmed/29615050
http://dx.doi.org/10.1186/s12936-018-2300-y
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