Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme

BACKGROUND: Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these enzymes need a native conformation stabilized by disulfide bonds, an elabor...

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Detalles Bibliográficos
Autores principales: Jalomo-Khayrova, Ekaterina, Mares, Rosa E., Muñoz, Patricia L. A., Meléndez-López, Samuel G., Rivero, Ignacio A., Ramos, Marco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883314/
https://www.ncbi.nlm.nih.gov/pubmed/29615011
http://dx.doi.org/10.1186/s12896-018-0429-y

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