Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD

INTRODUCTION: Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much...

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Autores principales: Kawai, Kazuaki, Kasai, Hiroshi, Li, Yun-Shan, Kawasaki, Yuya, Watanabe, Shintaro, Ohta, Masanori, Honda, Toru, Yamato, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883350/
https://www.ncbi.nlm.nih.gov/pubmed/29632621
http://dx.doi.org/10.1186/s41021-018-0095-2
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author Kawai, Kazuaki
Kasai, Hiroshi
Li, Yun-Shan
Kawasaki, Yuya
Watanabe, Shintaro
Ohta, Masanori
Honda, Toru
Yamato, Hiroshi
author_facet Kawai, Kazuaki
Kasai, Hiroshi
Li, Yun-Shan
Kawasaki, Yuya
Watanabe, Shintaro
Ohta, Masanori
Honda, Toru
Yamato, Hiroshi
author_sort Kawai, Kazuaki
collection PubMed
description INTRODUCTION: Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) as an oxidative stress marker in saliva, by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD). FINDINGS: The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The average level of 8-OHGua in saliva was 3.80 ng/mL in ordinary, non-smoking subjects. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers. CONCLUSIONS: Salivary 8-OHGua may be a useful noninvasive and promising oxidative stress biomarker.
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spelling pubmed-58833502018-04-09 Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD Kawai, Kazuaki Kasai, Hiroshi Li, Yun-Shan Kawasaki, Yuya Watanabe, Shintaro Ohta, Masanori Honda, Toru Yamato, Hiroshi Genes Environ Short Report INTRODUCTION: Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) as an oxidative stress marker in saliva, by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD). FINDINGS: The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The average level of 8-OHGua in saliva was 3.80 ng/mL in ordinary, non-smoking subjects. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers. CONCLUSIONS: Salivary 8-OHGua may be a useful noninvasive and promising oxidative stress biomarker. BioMed Central 2018-04-04 /pmc/articles/PMC5883350/ /pubmed/29632621 http://dx.doi.org/10.1186/s41021-018-0095-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Kawai, Kazuaki
Kasai, Hiroshi
Li, Yun-Shan
Kawasaki, Yuya
Watanabe, Shintaro
Ohta, Masanori
Honda, Toru
Yamato, Hiroshi
Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
title Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
title_full Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
title_fullStr Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
title_full_unstemmed Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
title_short Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
title_sort measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by hplc-ecd
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883350/
https://www.ncbi.nlm.nih.gov/pubmed/29632621
http://dx.doi.org/10.1186/s41021-018-0095-2
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