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The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
BACKGROUND: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883450/ https://www.ncbi.nlm.nih.gov/pubmed/29643668 http://dx.doi.org/10.4103/ijnm.IJNM_158_17 |
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author | Jafarpour, Seyed Masoud Safaei, Mehdi Mohseni, Mehran Salimian, Morteza Aliasgharzadeh, Akbar Fahood, Bagher |
author_facet | Jafarpour, Seyed Masoud Safaei, Mehdi Mohseni, Mehran Salimian, Morteza Aliasgharzadeh, Akbar Fahood, Bagher |
author_sort | Jafarpour, Seyed Masoud |
collection | PubMed |
description | BACKGROUND: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes. MATERIALS AND METHODS: First, 6-mL blood samples were taken from each of the five volunteers. After 1 h of preincubation with the antioxidants, a total of 20 μCi I-131/2 mL (blood + NaCl) was added to each sample, and then, the samples were reincubated for 1 h. Lymphocytes were separated and the mean DSB levels were measured for each sample through γ-H2AX assay to evaluate the effects of antioxidants. RESULTS: After 1-h incubation with I-131, the DSBs increased by 102.9% compared to the control group (0.343 vs. 0.169 DSB/cell; P = 0.00). Furthermore, compared to the control + I-131 group, curcumin and trehalose reduced the DSBs by 42% and 38%, respectively. There was a significant decrement (P = 0.00) in the levels of DSBs of the curcumin + I-131 and trehalose + I-131 subgroups compared to the control + I-131 subgroup. Furthermore, there was no significant relationship between the radioprotective effect of curcumin and trehalose (P = 0.95). CONCLUSION: The use of curcumin and trehalose as antioxidant can reduce the numbers of DSBs caused by I-131. Meanwhile, the radioprotective effect of curcumin was more than trehalose. |
format | Online Article Text |
id | pubmed-5883450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-58834502018-04-11 The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 Jafarpour, Seyed Masoud Safaei, Mehdi Mohseni, Mehran Salimian, Morteza Aliasgharzadeh, Akbar Fahood, Bagher Indian J Nucl Med Original Article BACKGROUND: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes. MATERIALS AND METHODS: First, 6-mL blood samples were taken from each of the five volunteers. After 1 h of preincubation with the antioxidants, a total of 20 μCi I-131/2 mL (blood + NaCl) was added to each sample, and then, the samples were reincubated for 1 h. Lymphocytes were separated and the mean DSB levels were measured for each sample through γ-H2AX assay to evaluate the effects of antioxidants. RESULTS: After 1-h incubation with I-131, the DSBs increased by 102.9% compared to the control group (0.343 vs. 0.169 DSB/cell; P = 0.00). Furthermore, compared to the control + I-131 group, curcumin and trehalose reduced the DSBs by 42% and 38%, respectively. There was a significant decrement (P = 0.00) in the levels of DSBs of the curcumin + I-131 and trehalose + I-131 subgroups compared to the control + I-131 subgroup. Furthermore, there was no significant relationship between the radioprotective effect of curcumin and trehalose (P = 0.95). CONCLUSION: The use of curcumin and trehalose as antioxidant can reduce the numbers of DSBs caused by I-131. Meanwhile, the radioprotective effect of curcumin was more than trehalose. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5883450/ /pubmed/29643668 http://dx.doi.org/10.4103/ijnm.IJNM_158_17 Text en Copyright: © 2018 Indian Journal of Nuclear Medicine http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Jafarpour, Seyed Masoud Safaei, Mehdi Mohseni, Mehran Salimian, Morteza Aliasgharzadeh, Akbar Fahood, Bagher The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 |
title | The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 |
title_full | The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 |
title_fullStr | The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 |
title_full_unstemmed | The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 |
title_short | The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 |
title_sort | radioprotective effects of curcumin and trehalose against genetic damage caused by i-131 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883450/ https://www.ncbi.nlm.nih.gov/pubmed/29643668 http://dx.doi.org/10.4103/ijnm.IJNM_158_17 |
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