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The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131

BACKGROUND: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and...

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Autores principales: Jafarpour, Seyed Masoud, Safaei, Mehdi, Mohseni, Mehran, Salimian, Morteza, Aliasgharzadeh, Akbar, Fahood, Bagher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883450/
https://www.ncbi.nlm.nih.gov/pubmed/29643668
http://dx.doi.org/10.4103/ijnm.IJNM_158_17
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author Jafarpour, Seyed Masoud
Safaei, Mehdi
Mohseni, Mehran
Salimian, Morteza
Aliasgharzadeh, Akbar
Fahood, Bagher
author_facet Jafarpour, Seyed Masoud
Safaei, Mehdi
Mohseni, Mehran
Salimian, Morteza
Aliasgharzadeh, Akbar
Fahood, Bagher
author_sort Jafarpour, Seyed Masoud
collection PubMed
description BACKGROUND: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes. MATERIALS AND METHODS: First, 6-mL blood samples were taken from each of the five volunteers. After 1 h of preincubation with the antioxidants, a total of 20 μCi I-131/2 mL (blood + NaCl) was added to each sample, and then, the samples were reincubated for 1 h. Lymphocytes were separated and the mean DSB levels were measured for each sample through γ-H2AX assay to evaluate the effects of antioxidants. RESULTS: After 1-h incubation with I-131, the DSBs increased by 102.9% compared to the control group (0.343 vs. 0.169 DSB/cell; P = 0.00). Furthermore, compared to the control + I-131 group, curcumin and trehalose reduced the DSBs by 42% and 38%, respectively. There was a significant decrement (P = 0.00) in the levels of DSBs of the curcumin + I-131 and trehalose + I-131 subgroups compared to the control + I-131 subgroup. Furthermore, there was no significant relationship between the radioprotective effect of curcumin and trehalose (P = 0.95). CONCLUSION: The use of curcumin and trehalose as antioxidant can reduce the numbers of DSBs caused by I-131. Meanwhile, the radioprotective effect of curcumin was more than trehalose.
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spelling pubmed-58834502018-04-11 The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131 Jafarpour, Seyed Masoud Safaei, Mehdi Mohseni, Mehran Salimian, Morteza Aliasgharzadeh, Akbar Fahood, Bagher Indian J Nucl Med Original Article BACKGROUND: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes. MATERIALS AND METHODS: First, 6-mL blood samples were taken from each of the five volunteers. After 1 h of preincubation with the antioxidants, a total of 20 μCi I-131/2 mL (blood + NaCl) was added to each sample, and then, the samples were reincubated for 1 h. Lymphocytes were separated and the mean DSB levels were measured for each sample through γ-H2AX assay to evaluate the effects of antioxidants. RESULTS: After 1-h incubation with I-131, the DSBs increased by 102.9% compared to the control group (0.343 vs. 0.169 DSB/cell; P = 0.00). Furthermore, compared to the control + I-131 group, curcumin and trehalose reduced the DSBs by 42% and 38%, respectively. There was a significant decrement (P = 0.00) in the levels of DSBs of the curcumin + I-131 and trehalose + I-131 subgroups compared to the control + I-131 subgroup. Furthermore, there was no significant relationship between the radioprotective effect of curcumin and trehalose (P = 0.95). CONCLUSION: The use of curcumin and trehalose as antioxidant can reduce the numbers of DSBs caused by I-131. Meanwhile, the radioprotective effect of curcumin was more than trehalose. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5883450/ /pubmed/29643668 http://dx.doi.org/10.4103/ijnm.IJNM_158_17 Text en Copyright: © 2018 Indian Journal of Nuclear Medicine http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Jafarpour, Seyed Masoud
Safaei, Mehdi
Mohseni, Mehran
Salimian, Morteza
Aliasgharzadeh, Akbar
Fahood, Bagher
The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
title The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
title_full The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
title_fullStr The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
title_full_unstemmed The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
title_short The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131
title_sort radioprotective effects of curcumin and trehalose against genetic damage caused by i-131
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883450/
https://www.ncbi.nlm.nih.gov/pubmed/29643668
http://dx.doi.org/10.4103/ijnm.IJNM_158_17
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