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Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid

BACKGROUND: The MiniR1–1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector. RESULTS: Nucleotide sequencing analysis shows that the MiniR1–1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication). The plasmid carries the repA, tap, copA an...

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Detalles Bibliográficos
Autores principales:	Yao, Yuan, Enkhtsetseg, Sukhbold, Odsbu, Ingvild, Fan, Lifei, Morigen, Morigen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883639/ https://www.ncbi.nlm.nih.gov/pubmed/29614952 http://dx.doi.org/10.1186/s12866-018-1162-3

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