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Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study

A large-scale study was conducted by multiple laboratories affiliated with the Japanese Environmental Mutagen Society and the Bacterial Mutagenicity Study Group to investigate possible proficiency indicators for the bacterial reverse mutation test with a preincubation procedure. Approximately 30 lab...

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Autores principales: Kato, Masayuki, Sugiyama, Kei-ichi, Fukushima, Toshiro, Miura, Yasuyoshi, Awogi, Takumi, Hikosaka, Shigetomo, Kawakami, Kumiko, Nakajima, Madoka, Nakamura, Masato, Sui, Hajime, Watanabe, Kumiko, Hakura, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883876/
https://www.ncbi.nlm.nih.gov/pubmed/29632622
http://dx.doi.org/10.1186/s41021-018-0096-1
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author Kato, Masayuki
Sugiyama, Kei-ichi
Fukushima, Toshiro
Miura, Yasuyoshi
Awogi, Takumi
Hikosaka, Shigetomo
Kawakami, Kumiko
Nakajima, Madoka
Nakamura, Masato
Sui, Hajime
Watanabe, Kumiko
Hakura, Atsushi
author_facet Kato, Masayuki
Sugiyama, Kei-ichi
Fukushima, Toshiro
Miura, Yasuyoshi
Awogi, Takumi
Hikosaka, Shigetomo
Kawakami, Kumiko
Nakajima, Madoka
Nakamura, Masato
Sui, Hajime
Watanabe, Kumiko
Hakura, Atsushi
author_sort Kato, Masayuki
collection PubMed
description A large-scale study was conducted by multiple laboratories affiliated with the Japanese Environmental Mutagen Society and the Bacterial Mutagenicity Study Group to investigate possible proficiency indicators for the bacterial reverse mutation test with a preincubation procedure. Approximately 30 laboratories generated negative and positive control count data and dose-response curves of the positive control articles for the bacterial reverse mutation test, with assays conducted annually from 2013 to 2016. Overall, the majority of the negative and positive control counts for Salmonella Typhimurium strains TA100, TA1535, TA98, and TA1537, and Escherichia coli strain WP2uvrA, with and without S9 mix, were within the range of the means ±2× standard deviation. The negative counts were normally distributed (strains TA100, TA98, and WP2uvrA) or followed Poisson distribution (strains TA1535 and TA1537), and the positive control counts for all strains were approximately normally distributed. In addition, the distribution of the negative and positive control counts was relatively constant over the 4 years. The number of revertant colonies increased in a dose-dependent linear or exponential fashion up to the recommended doses for the respective positive control articles in Japan. These data are valuable for determining the acceptance criteria and an estimation of the laboratory proficiency for the bacterial reverse mutation test. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s41021-018-0096-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-58838762018-04-09 Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study Kato, Masayuki Sugiyama, Kei-ichi Fukushima, Toshiro Miura, Yasuyoshi Awogi, Takumi Hikosaka, Shigetomo Kawakami, Kumiko Nakajima, Madoka Nakamura, Masato Sui, Hajime Watanabe, Kumiko Hakura, Atsushi Genes Environ Short Report A large-scale study was conducted by multiple laboratories affiliated with the Japanese Environmental Mutagen Society and the Bacterial Mutagenicity Study Group to investigate possible proficiency indicators for the bacterial reverse mutation test with a preincubation procedure. Approximately 30 laboratories generated negative and positive control count data and dose-response curves of the positive control articles for the bacterial reverse mutation test, with assays conducted annually from 2013 to 2016. Overall, the majority of the negative and positive control counts for Salmonella Typhimurium strains TA100, TA1535, TA98, and TA1537, and Escherichia coli strain WP2uvrA, with and without S9 mix, were within the range of the means ±2× standard deviation. The negative counts were normally distributed (strains TA100, TA98, and WP2uvrA) or followed Poisson distribution (strains TA1535 and TA1537), and the positive control counts for all strains were approximately normally distributed. In addition, the distribution of the negative and positive control counts was relatively constant over the 4 years. The number of revertant colonies increased in a dose-dependent linear or exponential fashion up to the recommended doses for the respective positive control articles in Japan. These data are valuable for determining the acceptance criteria and an estimation of the laboratory proficiency for the bacterial reverse mutation test. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s41021-018-0096-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-04 /pmc/articles/PMC5883876/ /pubmed/29632622 http://dx.doi.org/10.1186/s41021-018-0096-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Kato, Masayuki
Sugiyama, Kei-ichi
Fukushima, Toshiro
Miura, Yasuyoshi
Awogi, Takumi
Hikosaka, Shigetomo
Kawakami, Kumiko
Nakajima, Madoka
Nakamura, Masato
Sui, Hajime
Watanabe, Kumiko
Hakura, Atsushi
Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study
title Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study
title_full Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study
title_fullStr Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study
title_full_unstemmed Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study
title_short Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study
title_sort negative and positive control ranges in the bacterial reverse mutation test: jems/bms collaborative study
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883876/
https://www.ncbi.nlm.nih.gov/pubmed/29632622
http://dx.doi.org/10.1186/s41021-018-0096-1
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