Tunable Ampere phase plate for low dose imaging of biomolecular complexes

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented paralle...

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Autores principales: Tavabi, Amir H., Beleggia, Marco, Migunov, Vadim, Savenko, Alexey, Öktem, Ozan, Dunin-Borkowski, Rafal E., Pozzi, Giulio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884816/
https://www.ncbi.nlm.nih.gov/pubmed/29618785
http://dx.doi.org/10.1038/s41598-018-23100-3
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author Tavabi, Amir H.
Beleggia, Marco
Migunov, Vadim
Savenko, Alexey
Öktem, Ozan
Dunin-Borkowski, Rafal E.
Pozzi, Giulio
author_facet Tavabi, Amir H.
Beleggia, Marco
Migunov, Vadim
Savenko, Alexey
Öktem, Ozan
Dunin-Borkowski, Rafal E.
Pozzi, Giulio
author_sort Tavabi, Amir H.
collection PubMed
description A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere’s law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.
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spelling pubmed-58848162018-04-09 Tunable Ampere phase plate for low dose imaging of biomolecular complexes Tavabi, Amir H. Beleggia, Marco Migunov, Vadim Savenko, Alexey Öktem, Ozan Dunin-Borkowski, Rafal E. Pozzi, Giulio Sci Rep Article A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere’s law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules. Nature Publishing Group UK 2018-04-04 /pmc/articles/PMC5884816/ /pubmed/29618785 http://dx.doi.org/10.1038/s41598-018-23100-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tavabi, Amir H.
Beleggia, Marco
Migunov, Vadim
Savenko, Alexey
Öktem, Ozan
Dunin-Borkowski, Rafal E.
Pozzi, Giulio
Tunable Ampere phase plate for low dose imaging of biomolecular complexes
title Tunable Ampere phase plate for low dose imaging of biomolecular complexes
title_full Tunable Ampere phase plate for low dose imaging of biomolecular complexes
title_fullStr Tunable Ampere phase plate for low dose imaging of biomolecular complexes
title_full_unstemmed Tunable Ampere phase plate for low dose imaging of biomolecular complexes
title_short Tunable Ampere phase plate for low dose imaging of biomolecular complexes
title_sort tunable ampere phase plate for low dose imaging of biomolecular complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884816/
https://www.ncbi.nlm.nih.gov/pubmed/29618785
http://dx.doi.org/10.1038/s41598-018-23100-3
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