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A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain

The gray short-tailed opossum (Monodelphis domestica) is a small marsupial gaining recognition as a laboratory animal in biomedical research. Despite numerous studies on opossum neuroanatomy, a consistent and comprehensive neuroanatomical reference for this species is still missing. Here we present...

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Autores principales: Majka, Piotr, Chlodzinska, Natalia, Turlejski, Krzysztof, Banasik, Tomasz, Djavadian, Ruzanna L., Węglarz, Władysław P., Wójcik, Daniel K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884921/
https://www.ncbi.nlm.nih.gov/pubmed/29214509
http://dx.doi.org/10.1007/s00429-017-1540-x
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author Majka, Piotr
Chlodzinska, Natalia
Turlejski, Krzysztof
Banasik, Tomasz
Djavadian, Ruzanna L.
Węglarz, Władysław P.
Wójcik, Daniel K.
author_facet Majka, Piotr
Chlodzinska, Natalia
Turlejski, Krzysztof
Banasik, Tomasz
Djavadian, Ruzanna L.
Węglarz, Władysław P.
Wójcik, Daniel K.
author_sort Majka, Piotr
collection PubMed
description The gray short-tailed opossum (Monodelphis domestica) is a small marsupial gaining recognition as a laboratory animal in biomedical research. Despite numerous studies on opossum neuroanatomy, a consistent and comprehensive neuroanatomical reference for this species is still missing. Here we present the first three-dimensional, multimodal atlas of the Monodelphis opossum brain. It is based on four complementary imaging modalities: high resolution ex vivo magnetic resonance images, micro-computed tomography scans of the cranium, images of the face of the cutting block, and series of sections stained with the Nissl method and for myelinated fibers. Individual imaging modalities were reconstructed into a three-dimensional form and then registered to the MR image by means of affine and deformable registration routines. Based on a superimposition of the 3D images, 113 anatomical structures were demarcated and the volumes of individual regions were measured. The stereotaxic coordinate system was defined using a set of cranial landmarks: interaural line, bregma, and lambda, which allows for easy expression of any location within the brain with respect to the skull. The atlas is released under the Creative Commons license and available through various digital atlasing web services. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-017-1540-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-58849212018-04-10 A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain Majka, Piotr Chlodzinska, Natalia Turlejski, Krzysztof Banasik, Tomasz Djavadian, Ruzanna L. Węglarz, Władysław P. Wójcik, Daniel K. Brain Struct Funct Original Article The gray short-tailed opossum (Monodelphis domestica) is a small marsupial gaining recognition as a laboratory animal in biomedical research. Despite numerous studies on opossum neuroanatomy, a consistent and comprehensive neuroanatomical reference for this species is still missing. Here we present the first three-dimensional, multimodal atlas of the Monodelphis opossum brain. It is based on four complementary imaging modalities: high resolution ex vivo magnetic resonance images, micro-computed tomography scans of the cranium, images of the face of the cutting block, and series of sections stained with the Nissl method and for myelinated fibers. Individual imaging modalities were reconstructed into a three-dimensional form and then registered to the MR image by means of affine and deformable registration routines. Based on a superimposition of the 3D images, 113 anatomical structures were demarcated and the volumes of individual regions were measured. The stereotaxic coordinate system was defined using a set of cranial landmarks: interaural line, bregma, and lambda, which allows for easy expression of any location within the brain with respect to the skull. The atlas is released under the Creative Commons license and available through various digital atlasing web services. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-017-1540-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-12-06 2018 /pmc/articles/PMC5884921/ /pubmed/29214509 http://dx.doi.org/10.1007/s00429-017-1540-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Majka, Piotr
Chlodzinska, Natalia
Turlejski, Krzysztof
Banasik, Tomasz
Djavadian, Ruzanna L.
Węglarz, Władysław P.
Wójcik, Daniel K.
A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain
title A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain
title_full A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain
title_fullStr A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain
title_full_unstemmed A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain
title_short A three-dimensional stereotaxic atlas of the gray short-tailed opossum (Monodelphis domestica) brain
title_sort three-dimensional stereotaxic atlas of the gray short-tailed opossum (monodelphis domestica) brain
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884921/
https://www.ncbi.nlm.nih.gov/pubmed/29214509
http://dx.doi.org/10.1007/s00429-017-1540-x
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