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Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS

Xuebijing (XBJ) injection is an ethnomedicinal formula that has been widely used in the therapy of sepsis in China. However, the underlying theraputic mechanisms remain uninvestigated. In this research, a metabolomic method based on UHPLC-Q-Orbitrap HRMS was applied to make a holistic evaluation of...

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Autores principales: Zuo, Lihua, Zhou, Lin, Xu, Tanye, Li, Zhuolun, Liu, Liwei, Shi, Yingying, Kang, Jian, Gao, Guanmin, Du, Shuzhang, Sun, Zhi, Zhang, Xiaojian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884946/
https://www.ncbi.nlm.nih.gov/pubmed/29651245
http://dx.doi.org/10.3389/fphar.2018.00300
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author Zuo, Lihua
Zhou, Lin
Xu, Tanye
Li, Zhuolun
Liu, Liwei
Shi, Yingying
Kang, Jian
Gao, Guanmin
Du, Shuzhang
Sun, Zhi
Zhang, Xiaojian
author_facet Zuo, Lihua
Zhou, Lin
Xu, Tanye
Li, Zhuolun
Liu, Liwei
Shi, Yingying
Kang, Jian
Gao, Guanmin
Du, Shuzhang
Sun, Zhi
Zhang, Xiaojian
author_sort Zuo, Lihua
collection PubMed
description Xuebijing (XBJ) injection is an ethnomedicinal formula that has been widely used in the therapy of sepsis in China. However, the underlying theraputic mechanisms remain uninvestigated. In this research, a metabolomic method based on UHPLC-Q-Orbitrap HRMS was applied to make a holistic evaluation of XBJ on septic rats which were induced by the classical cecal ligation and puncture (CLP) operation. The plasma metabolic changes were profiled and evaluated by multivariate analytical (MVA) methods. In the results, a total of 41 differential metabolites were identified between CLP-operated group and sham-operated group, which were mainly involved in amino acid metabolism and lipid metabolism. After pathway analysis, it was finally discovered that the majority of the influenced metabolic pathways caused by sepsis mainly involved in energy metabolism, oxidative stress, and inflammation metabolism. When intervened by XBJ injection, 32 of the 41 disordered metabolites had been adjusted in reverse, which suggested that XBJ could mediate the abnormal metabolic pathways synergistically. In conclusion, the present study systematically investigated the efficacy and its underlying therapeutic mechanisms of XBJ on sepsis, while offering a new insight for the subsequent relevant exploration of other Chinese medicine at the same time.
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spelling pubmed-58849462018-04-12 Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS Zuo, Lihua Zhou, Lin Xu, Tanye Li, Zhuolun Liu, Liwei Shi, Yingying Kang, Jian Gao, Guanmin Du, Shuzhang Sun, Zhi Zhang, Xiaojian Front Pharmacol Pharmacology Xuebijing (XBJ) injection is an ethnomedicinal formula that has been widely used in the therapy of sepsis in China. However, the underlying theraputic mechanisms remain uninvestigated. In this research, a metabolomic method based on UHPLC-Q-Orbitrap HRMS was applied to make a holistic evaluation of XBJ on septic rats which were induced by the classical cecal ligation and puncture (CLP) operation. The plasma metabolic changes were profiled and evaluated by multivariate analytical (MVA) methods. In the results, a total of 41 differential metabolites were identified between CLP-operated group and sham-operated group, which were mainly involved in amino acid metabolism and lipid metabolism. After pathway analysis, it was finally discovered that the majority of the influenced metabolic pathways caused by sepsis mainly involved in energy metabolism, oxidative stress, and inflammation metabolism. When intervened by XBJ injection, 32 of the 41 disordered metabolites had been adjusted in reverse, which suggested that XBJ could mediate the abnormal metabolic pathways synergistically. In conclusion, the present study systematically investigated the efficacy and its underlying therapeutic mechanisms of XBJ on sepsis, while offering a new insight for the subsequent relevant exploration of other Chinese medicine at the same time. Frontiers Media S.A. 2018-03-29 /pmc/articles/PMC5884946/ /pubmed/29651245 http://dx.doi.org/10.3389/fphar.2018.00300 Text en Copyright © 2018 Zuo, Zhou, Xu, Li, Liu, Shi, Kang, Gao, Du, Sun and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zuo, Lihua
Zhou, Lin
Xu, Tanye
Li, Zhuolun
Liu, Liwei
Shi, Yingying
Kang, Jian
Gao, Guanmin
Du, Shuzhang
Sun, Zhi
Zhang, Xiaojian
Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS
title Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS
title_full Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS
title_fullStr Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS
title_full_unstemmed Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS
title_short Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS
title_sort antiseptic activity of ethnomedicinal xuebijing revealed by the metabolomics analysis using uhplc-q-orbitrap hrms
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884946/
https://www.ncbi.nlm.nih.gov/pubmed/29651245
http://dx.doi.org/10.3389/fphar.2018.00300
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