Cargando…

Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages

There is growing evidence that patients with Clostridiumdifficile-associated diarrhoea often acquire their infecting strain before hospital admission. Wastewater is known to be a potential source of surface water that is contaminated with C. difficile spores. Here, we describe a study that used geno...

Descripción completa

Detalles Bibliográficos
Autores principales: Moradigaravand, Danesh, Gouliouris, Theodore, Ludden, Catherine, Reuter, Sandra, Jamrozy, Dorota, Blane, Beth, Naydenova, Plamena, Judge, Kim, H. Aliyu, Sani, F. Hadjirin, Nazreen, A. Holmes, Mark, Török, Estée, M. Brown, Nicholas, Parkhill, Julian, Peacock, Sharon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5885014/
https://www.ncbi.nlm.nih.gov/pubmed/29498619
http://dx.doi.org/10.1099/mgen.0.000162
_version_ 1783311915510923264
author Moradigaravand, Danesh
Gouliouris, Theodore
Ludden, Catherine
Reuter, Sandra
Jamrozy, Dorota
Blane, Beth
Naydenova, Plamena
Judge, Kim
H. Aliyu, Sani
F. Hadjirin, Nazreen
A. Holmes, Mark
Török, Estée
M. Brown, Nicholas
Parkhill, Julian
Peacock, Sharon
author_facet Moradigaravand, Danesh
Gouliouris, Theodore
Ludden, Catherine
Reuter, Sandra
Jamrozy, Dorota
Blane, Beth
Naydenova, Plamena
Judge, Kim
H. Aliyu, Sani
F. Hadjirin, Nazreen
A. Holmes, Mark
Török, Estée
M. Brown, Nicholas
Parkhill, Julian
Peacock, Sharon
author_sort Moradigaravand, Danesh
collection PubMed
description There is growing evidence that patients with Clostridiumdifficile-associated diarrhoea often acquire their infecting strain before hospital admission. Wastewater is known to be a potential source of surface water that is contaminated with C. difficile spores. Here, we describe a study that used genome sequencing to compare C. difficile isolated from multiple wastewater treatment plants across the East of England and from patients with clinical disease at a major hospital in the same region. We confirmed that C. difficile from 65 patients were highly diverse and that most cases were not linked to other active cases in the hospital. In total, 186 C. difficile isolates were isolated from effluent water obtained from 18 municipal treatment plants at the point of release into the environment. Whole genome comparisons of clinical and environmental isolates demonstrated highly related populations, and confirmed extensive release of toxigenic C. difficile into surface waters. An analysis based on multilocus sequence types (STs) identified 19 distinct STs in the clinical collection and 38 STs in the wastewater collection, with 13 of 44 STs common to both clinical and wastewater collections. Furthermore, we identified five pairs of highly similar isolates (≤2 SNPs different in the core genome) in clinical and wastewater collections. Strategies to control community acquisition should consider the need for bacterial control of treated wastewater.
format Online
Article
Text
id pubmed-5885014
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-58850142018-04-05 Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages Moradigaravand, Danesh Gouliouris, Theodore Ludden, Catherine Reuter, Sandra Jamrozy, Dorota Blane, Beth Naydenova, Plamena Judge, Kim H. Aliyu, Sani F. Hadjirin, Nazreen A. Holmes, Mark Török, Estée M. Brown, Nicholas Parkhill, Julian Peacock, Sharon Microb Genom Research Article There is growing evidence that patients with Clostridiumdifficile-associated diarrhoea often acquire their infecting strain before hospital admission. Wastewater is known to be a potential source of surface water that is contaminated with C. difficile spores. Here, we describe a study that used genome sequencing to compare C. difficile isolated from multiple wastewater treatment plants across the East of England and from patients with clinical disease at a major hospital in the same region. We confirmed that C. difficile from 65 patients were highly diverse and that most cases were not linked to other active cases in the hospital. In total, 186 C. difficile isolates were isolated from effluent water obtained from 18 municipal treatment plants at the point of release into the environment. Whole genome comparisons of clinical and environmental isolates demonstrated highly related populations, and confirmed extensive release of toxigenic C. difficile into surface waters. An analysis based on multilocus sequence types (STs) identified 19 distinct STs in the clinical collection and 38 STs in the wastewater collection, with 13 of 44 STs common to both clinical and wastewater collections. Furthermore, we identified five pairs of highly similar isolates (≤2 SNPs different in the core genome) in clinical and wastewater collections. Strategies to control community acquisition should consider the need for bacterial control of treated wastewater. Microbiology Society 2018-03-02 /pmc/articles/PMC5885014/ /pubmed/29498619 http://dx.doi.org/10.1099/mgen.0.000162 Text en http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Moradigaravand, Danesh
Gouliouris, Theodore
Ludden, Catherine
Reuter, Sandra
Jamrozy, Dorota
Blane, Beth
Naydenova, Plamena
Judge, Kim
H. Aliyu, Sani
F. Hadjirin, Nazreen
A. Holmes, Mark
Török, Estée
M. Brown, Nicholas
Parkhill, Julian
Peacock, Sharon
Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages
title Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages
title_full Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages
title_fullStr Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages
title_full_unstemmed Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages
title_short Genomic survey of Clostridium difficile reservoirs in the East of England implicates environmental contamination of wastewater treatment plants by clinical lineages
title_sort genomic survey of clostridium difficile reservoirs in the east of england implicates environmental contamination of wastewater treatment plants by clinical lineages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5885014/
https://www.ncbi.nlm.nih.gov/pubmed/29498619
http://dx.doi.org/10.1099/mgen.0.000162
work_keys_str_mv AT moradigaravanddanesh genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT gouliouristheodore genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT luddencatherine genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT reutersandra genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT jamrozydorota genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT blanebeth genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT naydenovaplamena genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT judgekim genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT haliyusani genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT fhadjirinnazreen genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT aholmesmark genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT torokestee genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT mbrownnicholas genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT parkhilljulian genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages
AT peacocksharon genomicsurveyofclostridiumdifficilereservoirsintheeastofenglandimplicatesenvironmentalcontaminationofwastewatertreatmentplantsbyclinicallineages