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Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex

Dopamine receptor expression is correlated with certain types of cancers, including lung, breast and colon cancers. In this study, we report luminescent iridium(iii) complexes (11–14) as intracellular dopamine receptor (D1R/D2R) cell imaging agents. Complexes 11 and 13, which are conjugated with a d...

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Autores principales: Vellaisamy, Kasipandi, Li, Guodong, Ko, Chung-Nga, Zhong, Hai-Jing, Fatima, Sarwat, Kwan, Hiu-Yee, Wong, Chun-Yuen, Kwong, Wai-Jing, Tan, Weihong, Leung, Chung-Hang, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5885777/
https://www.ncbi.nlm.nih.gov/pubmed/29675156
http://dx.doi.org/10.1039/c7sc04798c
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author Vellaisamy, Kasipandi
Li, Guodong
Ko, Chung-Nga
Zhong, Hai-Jing
Fatima, Sarwat
Kwan, Hiu-Yee
Wong, Chun-Yuen
Kwong, Wai-Jing
Tan, Weihong
Leung, Chung-Hang
Ma, Dik-Lung
author_facet Vellaisamy, Kasipandi
Li, Guodong
Ko, Chung-Nga
Zhong, Hai-Jing
Fatima, Sarwat
Kwan, Hiu-Yee
Wong, Chun-Yuen
Kwong, Wai-Jing
Tan, Weihong
Leung, Chung-Hang
Ma, Dik-Lung
author_sort Vellaisamy, Kasipandi
collection PubMed
description Dopamine receptor expression is correlated with certain types of cancers, including lung, breast and colon cancers. In this study, we report luminescent iridium(iii) complexes (11–14) as intracellular dopamine receptor (D1R/D2R) cell imaging agents. Complexes 11 and 13, which are conjugated with a dopamine receptor agonist, showed superior cell imaging characteristics, high stability and low cytotoxicity (>100 μM) in A549 lung cancer cells. siRNA knockdown and dopamine competitive assays indicated that complexes 11 and 13 could selectively bind to dopamine receptors (D1R/D2R) in A549 cells. Fluorescence lifetime microscopy demonstrated that complex 13 has a longer luminescence lifetime at the wavelength of 560–650 nm than DAPI and other chromophores in biological fluids. The long luminescence lifetime of complex 13 not only provides an opportunity for efficient dopamine receptor tracking in biological media, but also enables the temporal separation of the probe signal from the intense background signal by fluorescence lifetime microscopy for efficient analysis. Complex 13 also shows high photostability, which could allow it to be employed for long-term cellular imaging. Furthermore, complex 13 could selectively track the internalization process of dopamine receptors (D1R/D2R) in living cells. To the best of our knowledge, complex 13 is the first metal-based compound that has been used to monitor intracellular dopamine receptors in living cells.
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spelling pubmed-58857772018-04-19 Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex Vellaisamy, Kasipandi Li, Guodong Ko, Chung-Nga Zhong, Hai-Jing Fatima, Sarwat Kwan, Hiu-Yee Wong, Chun-Yuen Kwong, Wai-Jing Tan, Weihong Leung, Chung-Hang Ma, Dik-Lung Chem Sci Chemistry Dopamine receptor expression is correlated with certain types of cancers, including lung, breast and colon cancers. In this study, we report luminescent iridium(iii) complexes (11–14) as intracellular dopamine receptor (D1R/D2R) cell imaging agents. Complexes 11 and 13, which are conjugated with a dopamine receptor agonist, showed superior cell imaging characteristics, high stability and low cytotoxicity (>100 μM) in A549 lung cancer cells. siRNA knockdown and dopamine competitive assays indicated that complexes 11 and 13 could selectively bind to dopamine receptors (D1R/D2R) in A549 cells. Fluorescence lifetime microscopy demonstrated that complex 13 has a longer luminescence lifetime at the wavelength of 560–650 nm than DAPI and other chromophores in biological fluids. The long luminescence lifetime of complex 13 not only provides an opportunity for efficient dopamine receptor tracking in biological media, but also enables the temporal separation of the probe signal from the intense background signal by fluorescence lifetime microscopy for efficient analysis. Complex 13 also shows high photostability, which could allow it to be employed for long-term cellular imaging. Furthermore, complex 13 could selectively track the internalization process of dopamine receptors (D1R/D2R) in living cells. To the best of our knowledge, complex 13 is the first metal-based compound that has been used to monitor intracellular dopamine receptors in living cells. Royal Society of Chemistry 2017-12-19 /pmc/articles/PMC5885777/ /pubmed/29675156 http://dx.doi.org/10.1039/c7sc04798c Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Vellaisamy, Kasipandi
Li, Guodong
Ko, Chung-Nga
Zhong, Hai-Jing
Fatima, Sarwat
Kwan, Hiu-Yee
Wong, Chun-Yuen
Kwong, Wai-Jing
Tan, Weihong
Leung, Chung-Hang
Ma, Dik-Lung
Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
title Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
title_full Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
title_fullStr Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
title_full_unstemmed Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
title_short Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
title_sort cell imaging of dopamine receptor using agonist labeling iridium(iii) complex
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5885777/
https://www.ncbi.nlm.nih.gov/pubmed/29675156
http://dx.doi.org/10.1039/c7sc04798c
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