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Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific

OBJECTIVE: The study aims to explore long-term dietary effects on increases in body mass and fat depot enlargement through the recruitment of early in life labeled progenitor cells to the adipolineage. DESIGN AND METHODS: Neonate male C57BL/6J (B6) mice were injected intraperitoneally with BrdU. Fro...

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Autores principales: Gawronska-Kozak, B., Staszkiewicz, J., Gimble, J. M., Kirk-Ballard, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5886012/
https://www.ncbi.nlm.nih.gov/pubmed/24795999
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author Gawronska-Kozak, B.
Staszkiewicz, J.
Gimble, J. M.
Kirk-Ballard, H.
author_facet Gawronska-Kozak, B.
Staszkiewicz, J.
Gimble, J. M.
Kirk-Ballard, H.
author_sort Gawronska-Kozak, B.
collection PubMed
description OBJECTIVE: The study aims to explore long-term dietary effects on increases in body mass and fat depot enlargement through the recruitment of early in life labeled progenitor cells to the adipolineage. DESIGN AND METHODS: Neonate male C57BL/6J (B6) mice were injected intraperitoneally with BrdU. From 4 until 30 weeks of age they were fed either low fat diet (LFD) or high fat diet (HFD). BrdU-labeled cells were analyzed by flow cytometric and immunohistochemical assays after 10 days and 4, 8, 16 and 30 weeks. RESULTS: Mice fed HFD were heavier than mice fed LFD with the most dramatic disparity recorded between week 16 and 30. BrdU-bearing cells showed the decrease in the percentage content of labeled cells in inguinal (iWAT), epididymal (eWAT) and bone marrow (BM) tissues, regardless diets. However, iWAT collected from animals on HFD showed significant increase in labeled-cells at week 16th, which coincides with robust increase in inguinal but not epididymal fat weight between 16 and 30 weeks age. CONCLUSIONS: Cells labeled with BrdU during neonate life of B6 mice persist in fat tissues for long period of time and are recruited to the adipocyte lineage in a favorable (obesogenic) environment in iWAT but not in eWAT.
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spelling pubmed-58860122018-04-05 Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific Gawronska-Kozak, B. Staszkiewicz, J. Gimble, J. M. Kirk-Ballard, H. Obesity (Silver Spring) Article OBJECTIVE: The study aims to explore long-term dietary effects on increases in body mass and fat depot enlargement through the recruitment of early in life labeled progenitor cells to the adipolineage. DESIGN AND METHODS: Neonate male C57BL/6J (B6) mice were injected intraperitoneally with BrdU. From 4 until 30 weeks of age they were fed either low fat diet (LFD) or high fat diet (HFD). BrdU-labeled cells were analyzed by flow cytometric and immunohistochemical assays after 10 days and 4, 8, 16 and 30 weeks. RESULTS: Mice fed HFD were heavier than mice fed LFD with the most dramatic disparity recorded between week 16 and 30. BrdU-bearing cells showed the decrease in the percentage content of labeled cells in inguinal (iWAT), epididymal (eWAT) and bone marrow (BM) tissues, regardless diets. However, iWAT collected from animals on HFD showed significant increase in labeled-cells at week 16th, which coincides with robust increase in inguinal but not epididymal fat weight between 16 and 30 weeks age. CONCLUSIONS: Cells labeled with BrdU during neonate life of B6 mice persist in fat tissues for long period of time and are recruited to the adipocyte lineage in a favorable (obesogenic) environment in iWAT but not in eWAT. 2014-04 /pmc/articles/PMC5886012/ /pubmed/24795999 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Gawronska-Kozak, B.
Staszkiewicz, J.
Gimble, J. M.
Kirk-Ballard, H.
Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific
title Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific
title_full Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific
title_fullStr Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific
title_full_unstemmed Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific
title_short Recruitment of fat cell precursors during long-term high fat diet in C57BL/6J mice is fat depot specific
title_sort recruitment of fat cell precursors during long-term high fat diet in c57bl/6j mice is fat depot specific
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5886012/
https://www.ncbi.nlm.nih.gov/pubmed/24795999
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