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Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108

Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated histone tails that consequently interact more closely with DNA, leading to chromatin state refractory to transcription. Zea mays HDA108 belongs to the Rpd3/HDA1 HDAC family and is ubiquitously expressed during develop...

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Autores principales: Forestan, Cristian, Farinati, Silvia, Rouster, Jacques, Lassagne, Hervé, Lauria, Massimiliano, Dal Ferro, Nicola, Varotto, Serena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887141/
https://www.ncbi.nlm.nih.gov/pubmed/29382649
http://dx.doi.org/10.1534/genetics.117.300625
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author Forestan, Cristian
Farinati, Silvia
Rouster, Jacques
Lassagne, Hervé
Lauria, Massimiliano
Dal Ferro, Nicola
Varotto, Serena
author_facet Forestan, Cristian
Farinati, Silvia
Rouster, Jacques
Lassagne, Hervé
Lauria, Massimiliano
Dal Ferro, Nicola
Varotto, Serena
author_sort Forestan, Cristian
collection PubMed
description Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated histone tails that consequently interact more closely with DNA, leading to chromatin state refractory to transcription. Zea mays HDA108 belongs to the Rpd3/HDA1 HDAC family and is ubiquitously expressed during development. The newly isolated hda108/hda108 insertional mutant exhibited many developmental defects: significant reduction in plant height, alterations of shoot and leaf development, and alterations of inflorescence patterning and fertility. Western blot analyses and immunolocalization experiments revealed an evident increase in histone acetylation, accompanied by a marked reduction in H3K9 dimethylation, in mutant nuclei. The DNA methylation status, in the CHG sequence context, and the transcript level of ribosomal sequences were also affected in hda108 mutants, while enrichment in H3 and H4 acetylation characterizes both repetitive and nonrepetitive transcriptional up-regulated loci. RNA-Seq of both young leaf and anthers indicated that transcription factor expression is highly affected and that the pollen developmental program is disrupted in hda108 mutants. Crosses between hda108/hda108 and epiregulator mutants did not produce any double mutant progeny indicating possible genetic interactions of HDA108 with distinct epigenetic pathways. Our findings indicate that HDA108 is directly involved in regulation of maize development, fertility, and epigenetic regulation of genome activity.
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spelling pubmed-58871412018-04-06 Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108 Forestan, Cristian Farinati, Silvia Rouster, Jacques Lassagne, Hervé Lauria, Massimiliano Dal Ferro, Nicola Varotto, Serena Genetics Investigations Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated histone tails that consequently interact more closely with DNA, leading to chromatin state refractory to transcription. Zea mays HDA108 belongs to the Rpd3/HDA1 HDAC family and is ubiquitously expressed during development. The newly isolated hda108/hda108 insertional mutant exhibited many developmental defects: significant reduction in plant height, alterations of shoot and leaf development, and alterations of inflorescence patterning and fertility. Western blot analyses and immunolocalization experiments revealed an evident increase in histone acetylation, accompanied by a marked reduction in H3K9 dimethylation, in mutant nuclei. The DNA methylation status, in the CHG sequence context, and the transcript level of ribosomal sequences were also affected in hda108 mutants, while enrichment in H3 and H4 acetylation characterizes both repetitive and nonrepetitive transcriptional up-regulated loci. RNA-Seq of both young leaf and anthers indicated that transcription factor expression is highly affected and that the pollen developmental program is disrupted in hda108 mutants. Crosses between hda108/hda108 and epiregulator mutants did not produce any double mutant progeny indicating possible genetic interactions of HDA108 with distinct epigenetic pathways. Our findings indicate that HDA108 is directly involved in regulation of maize development, fertility, and epigenetic regulation of genome activity. Genetics Society of America 2018-04 2018-01-29 /pmc/articles/PMC5887141/ /pubmed/29382649 http://dx.doi.org/10.1534/genetics.117.300625 Text en Copyright © 2018 by the Genetics Society of America Available freely online through the author-supported open access option.
spellingShingle Investigations
Forestan, Cristian
Farinati, Silvia
Rouster, Jacques
Lassagne, Hervé
Lauria, Massimiliano
Dal Ferro, Nicola
Varotto, Serena
Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108
title Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108
title_full Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108
title_fullStr Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108
title_full_unstemmed Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108
title_short Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108
title_sort control of maize vegetative and reproductive development, fertility, and rrnas silencing by histone deacetylase 108
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887141/
https://www.ncbi.nlm.nih.gov/pubmed/29382649
http://dx.doi.org/10.1534/genetics.117.300625
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