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Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells
BACKGROUND: Cathepsin S is a cysteine protease, which is expressed in human periodontal ligament (PDL) cells under inflammatory and infectious conditions. This in vitro study was established to investigate the effect of cathepsin S on PDL cell wound closure. METHODS: An in vitro wound healing assay...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887187/ https://www.ncbi.nlm.nih.gov/pubmed/29622023 http://dx.doi.org/10.1186/s12903-018-0518-2 |
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author | Memmert, Svenja Nokhbehsaim, Marjan Damanaki, Anna Nogueira, Andressa V. B. Papadopoulou, Alexandra K. Piperi, Christina Basdra, Efthimia K. Rath-Deschner, Birgit Götz, Werner Cirelli, Joni A. Jäger, Andreas Deschner, James |
author_facet | Memmert, Svenja Nokhbehsaim, Marjan Damanaki, Anna Nogueira, Andressa V. B. Papadopoulou, Alexandra K. Piperi, Christina Basdra, Efthimia K. Rath-Deschner, Birgit Götz, Werner Cirelli, Joni A. Jäger, Andreas Deschner, James |
author_sort | Memmert, Svenja |
collection | PubMed |
description | BACKGROUND: Cathepsin S is a cysteine protease, which is expressed in human periodontal ligament (PDL) cells under inflammatory and infectious conditions. This in vitro study was established to investigate the effect of cathepsin S on PDL cell wound closure. METHODS: An in vitro wound healing assay was used to monitor wound closure in wounded PDL cell monolayers for 72 h in the presence and absence of cathepsin S. In addition, the effects of cathepsin S on specific markers for apoptosis and proliferation were studied at transcriptional level. Changes in the proliferation rate due to cathepsin S stimulation were analyzed by an XTT assay, and the actions of cathepsin S on cell migration were investigated via live cell tracking. Additionally, PDL cell monolayers were treated with a toll-like receptor 2 agonist in the presence and absence of a cathepsin inhibitor to examine if periodontal bacteria can alter wound closure via cathepsins. RESULTS: Cathepsin S enhanced significantly the in vitro wound healing rate by inducing proliferation and by increasing the speed of cell migration, but had no effect on apoptosis. Moreover, the toll-like receptor 2 agonist enhanced significantly the wound closure and this stimulatory effect was dependent on cathepsins. CONCLUSIONS: Our findings provide original evidence that cathepsin S stimulates PDL cell proliferation and migration and, thereby, wound closure, suggesting that this cysteine protease might play a critical role in periodontal remodeling and healing. In addition, cathepsins might be exploited by periodontal bacteria to regulate critical PDL cell functions. |
format | Online Article Text |
id | pubmed-5887187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58871872018-04-09 Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells Memmert, Svenja Nokhbehsaim, Marjan Damanaki, Anna Nogueira, Andressa V. B. Papadopoulou, Alexandra K. Piperi, Christina Basdra, Efthimia K. Rath-Deschner, Birgit Götz, Werner Cirelli, Joni A. Jäger, Andreas Deschner, James BMC Oral Health Research Article BACKGROUND: Cathepsin S is a cysteine protease, which is expressed in human periodontal ligament (PDL) cells under inflammatory and infectious conditions. This in vitro study was established to investigate the effect of cathepsin S on PDL cell wound closure. METHODS: An in vitro wound healing assay was used to monitor wound closure in wounded PDL cell monolayers for 72 h in the presence and absence of cathepsin S. In addition, the effects of cathepsin S on specific markers for apoptosis and proliferation were studied at transcriptional level. Changes in the proliferation rate due to cathepsin S stimulation were analyzed by an XTT assay, and the actions of cathepsin S on cell migration were investigated via live cell tracking. Additionally, PDL cell monolayers were treated with a toll-like receptor 2 agonist in the presence and absence of a cathepsin inhibitor to examine if periodontal bacteria can alter wound closure via cathepsins. RESULTS: Cathepsin S enhanced significantly the in vitro wound healing rate by inducing proliferation and by increasing the speed of cell migration, but had no effect on apoptosis. Moreover, the toll-like receptor 2 agonist enhanced significantly the wound closure and this stimulatory effect was dependent on cathepsins. CONCLUSIONS: Our findings provide original evidence that cathepsin S stimulates PDL cell proliferation and migration and, thereby, wound closure, suggesting that this cysteine protease might play a critical role in periodontal remodeling and healing. In addition, cathepsins might be exploited by periodontal bacteria to regulate critical PDL cell functions. BioMed Central 2018-04-05 /pmc/articles/PMC5887187/ /pubmed/29622023 http://dx.doi.org/10.1186/s12903-018-0518-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Memmert, Svenja Nokhbehsaim, Marjan Damanaki, Anna Nogueira, Andressa V. B. Papadopoulou, Alexandra K. Piperi, Christina Basdra, Efthimia K. Rath-Deschner, Birgit Götz, Werner Cirelli, Joni A. Jäger, Andreas Deschner, James Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells |
title | Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells |
title_full | Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells |
title_fullStr | Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells |
title_full_unstemmed | Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells |
title_short | Role of cathepsin S In periodontal wound healing–an in vitro study on human PDL cells |
title_sort | role of cathepsin s in periodontal wound healing–an in vitro study on human pdl cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887187/ https://www.ncbi.nlm.nih.gov/pubmed/29622023 http://dx.doi.org/10.1186/s12903-018-0518-2 |
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