Cargando…

Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women

BACKGROUND: The identification of variants in the nicotinic acetylcholine receptor (nAChR) subunit genes associated with smoking phenotypes are increasingly important for prevention and treatment of nicotine dependence. In the context of personalized medicine, the aims of this study were to evaluate...

Descripción completa

Detalles Bibliográficos
Autores principales: Tomaz, Paulo Roberto Xavier, Santos, Juliana Rocha, Scholz, Jaqueline, Abe, Tânia Ogawa, Gaya, Patrícia Viviane, Negrão, André Brooking, Krieger, José Eduardo, Pereira, Alexandre Costa, Santos, Paulo Caleb Júnior Lima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887212/
https://www.ncbi.nlm.nih.gov/pubmed/29621993
http://dx.doi.org/10.1186/s12881-018-0571-3
_version_ 1783312249666928640
author Tomaz, Paulo Roberto Xavier
Santos, Juliana Rocha
Scholz, Jaqueline
Abe, Tânia Ogawa
Gaya, Patrícia Viviane
Negrão, André Brooking
Krieger, José Eduardo
Pereira, Alexandre Costa
Santos, Paulo Caleb Júnior Lima
author_facet Tomaz, Paulo Roberto Xavier
Santos, Juliana Rocha
Scholz, Jaqueline
Abe, Tânia Ogawa
Gaya, Patrícia Viviane
Negrão, André Brooking
Krieger, José Eduardo
Pereira, Alexandre Costa
Santos, Paulo Caleb Júnior Lima
author_sort Tomaz, Paulo Roberto Xavier
collection PubMed
description BACKGROUND: The identification of variants in the nicotinic acetylcholine receptor (nAChR) subunit genes associated with smoking phenotypes are increasingly important for prevention and treatment of nicotine dependence. In the context of personalized medicine, the aims of this study were to evaluate whether cholinergic receptor nicotinic alpha 2 (CHRNA2), cholinergic receptor nicotinic alpha 3 (CHRNA3), cholinergic receptor nicotinic alpha 5 (CHRNA5) and cholinergic receptor nicotinic beta 3 (CHRNB3) polymorphisms were associated with nicotine dependence severity, and to investigate possible pharmacogenetics markers of smoking cessation treatment. METHODS: This study cohort enrolled 1049 smoking patients who received pharmacological treatment (varenicline, varenicline plus bupropion, bupropion plus/or nicotine replacement therapy). Smoking cessation success was considered for patients who completed 6 months of continuous abstinence. Fagerström test for nicotine dependence (FTND) and Issa situational smoking scores (Issa score) were analyzed for nicotine dependence. CHRNA2 (rs2472553), CHRNA3 (rs1051730), CHRNA5 (rs16969968 and rs2036527) and CHRNB3 (rs6474413) polymorphisms were genotyped by high resolution melting analysis. RESULTS: Females with GA and AA genotypes for CHRNA5 rs16969968 and rs2036527 polymorphisms had higher success rate in smoking cessation treatment: 44.0% and 56.3% (rs16969968), 41.5% and 56.5% (rs2036527), respectively, compared with carriers of the GG genotypes: 35.7% (rs16969968), 34.8% (rs2036527), (P = 0.03, n = 389; P = 0.01, n = 391). The GA or AA genotypes for the rs16969968 and rs2036527 were associated with higher odds ratio for success in women (OR = 1.63; 95% CI = 1.04 to 2.54; P = 0.03 and OR = 1.59, 95% CI = 1.02 to 2.48; P = 0.04; respectively). We did not find association of these polymorphisms with nicotine dependence related scores. Polymorphisms in the CHRNA2, CHRNA3 and CHRNB3 genes were not associated with the phenotypes studied. CONCLUSION: CHRNA5 rs16969968 and rs2036527 were associated with higher success rate in the smoking cessation treatment in women. These findings might contribute to advances in personalized medicine. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12881-018-0571-3) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5887212
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-58872122018-04-09 Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women Tomaz, Paulo Roberto Xavier Santos, Juliana Rocha Scholz, Jaqueline Abe, Tânia Ogawa Gaya, Patrícia Viviane Negrão, André Brooking Krieger, José Eduardo Pereira, Alexandre Costa Santos, Paulo Caleb Júnior Lima BMC Med Genet Research Article BACKGROUND: The identification of variants in the nicotinic acetylcholine receptor (nAChR) subunit genes associated with smoking phenotypes are increasingly important for prevention and treatment of nicotine dependence. In the context of personalized medicine, the aims of this study were to evaluate whether cholinergic receptor nicotinic alpha 2 (CHRNA2), cholinergic receptor nicotinic alpha 3 (CHRNA3), cholinergic receptor nicotinic alpha 5 (CHRNA5) and cholinergic receptor nicotinic beta 3 (CHRNB3) polymorphisms were associated with nicotine dependence severity, and to investigate possible pharmacogenetics markers of smoking cessation treatment. METHODS: This study cohort enrolled 1049 smoking patients who received pharmacological treatment (varenicline, varenicline plus bupropion, bupropion plus/or nicotine replacement therapy). Smoking cessation success was considered for patients who completed 6 months of continuous abstinence. Fagerström test for nicotine dependence (FTND) and Issa situational smoking scores (Issa score) were analyzed for nicotine dependence. CHRNA2 (rs2472553), CHRNA3 (rs1051730), CHRNA5 (rs16969968 and rs2036527) and CHRNB3 (rs6474413) polymorphisms were genotyped by high resolution melting analysis. RESULTS: Females with GA and AA genotypes for CHRNA5 rs16969968 and rs2036527 polymorphisms had higher success rate in smoking cessation treatment: 44.0% and 56.3% (rs16969968), 41.5% and 56.5% (rs2036527), respectively, compared with carriers of the GG genotypes: 35.7% (rs16969968), 34.8% (rs2036527), (P = 0.03, n = 389; P = 0.01, n = 391). The GA or AA genotypes for the rs16969968 and rs2036527 were associated with higher odds ratio for success in women (OR = 1.63; 95% CI = 1.04 to 2.54; P = 0.03 and OR = 1.59, 95% CI = 1.02 to 2.48; P = 0.04; respectively). We did not find association of these polymorphisms with nicotine dependence related scores. Polymorphisms in the CHRNA2, CHRNA3 and CHRNB3 genes were not associated with the phenotypes studied. CONCLUSION: CHRNA5 rs16969968 and rs2036527 were associated with higher success rate in the smoking cessation treatment in women. These findings might contribute to advances in personalized medicine. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12881-018-0571-3) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-05 /pmc/articles/PMC5887212/ /pubmed/29621993 http://dx.doi.org/10.1186/s12881-018-0571-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Tomaz, Paulo Roberto Xavier
Santos, Juliana Rocha
Scholz, Jaqueline
Abe, Tânia Ogawa
Gaya, Patrícia Viviane
Negrão, André Brooking
Krieger, José Eduardo
Pereira, Alexandre Costa
Santos, Paulo Caleb Júnior Lima
Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
title Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
title_full Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
title_fullStr Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
title_full_unstemmed Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
title_short Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
title_sort cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887212/
https://www.ncbi.nlm.nih.gov/pubmed/29621993
http://dx.doi.org/10.1186/s12881-018-0571-3
work_keys_str_mv AT tomazpaulorobertoxavier cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT santosjulianarocha cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT scholzjaqueline cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT abetaniaogawa cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT gayapatriciaviviane cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT negraoandrebrooking cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT kriegerjoseeduardo cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT pereiraalexandrecosta cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen
AT santospaulocalebjuniorlima cholinergicreceptornicotinicalpha5subunitpolymorphismsareassociatedwithsmokingcessationsuccessinwomen