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Estimation and quantification of human DNA in dental calculus: A pilot study

CONTEXT: Identification using DNA has proved its accuracy multiple times in the field of forensic investigations. Investigators usually rely on either teeth or bone as the DNA reservoirs. However, there are instances where the skeletal or dental remains are not available or not preserved properly. M...

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Autores principales: Singh, Udita, Goel, Saurabh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887638/
https://www.ncbi.nlm.nih.gov/pubmed/29657492
http://dx.doi.org/10.4103/jfo.jfds_94_16
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author Singh, Udita
Goel, Saurabh
author_facet Singh, Udita
Goel, Saurabh
author_sort Singh, Udita
collection PubMed
description CONTEXT: Identification using DNA has proved its accuracy multiple times in the field of forensic investigations. Investigators usually rely on either teeth or bone as the DNA reservoirs. However, there are instances where the skeletal or dental remains are not available or not preserved properly. Moreover, due to religious beliefs, the family members of the dead do not allow the investigating team to damage the remains for the sole purpose of identification. AIM: To investigate the presence of human DNA in dental calculus and to quantify the amount, if present. MATERIALS AND METHODS: This prospective single-blinded pilot study included twenty subjects selected from the patients visiting a dental college. The samples of dental calculus were collected from the thickest portion of calculus deposited on the lingual surfaces of mandibular incisors. These samples were decontaminated and subjected to gel electrophoresis for DNA extraction. RESULTS: DNA was found in 85% cases. The amount of DNA varied from 21 to 37 μg/ml of dental calculus. CONCLUSION: Dental calculus is a rich reservoir of human DNA.
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spelling pubmed-58876382018-04-13 Estimation and quantification of human DNA in dental calculus: A pilot study Singh, Udita Goel, Saurabh J Forensic Dent Sci Original Article CONTEXT: Identification using DNA has proved its accuracy multiple times in the field of forensic investigations. Investigators usually rely on either teeth or bone as the DNA reservoirs. However, there are instances where the skeletal or dental remains are not available or not preserved properly. Moreover, due to religious beliefs, the family members of the dead do not allow the investigating team to damage the remains for the sole purpose of identification. AIM: To investigate the presence of human DNA in dental calculus and to quantify the amount, if present. MATERIALS AND METHODS: This prospective single-blinded pilot study included twenty subjects selected from the patients visiting a dental college. The samples of dental calculus were collected from the thickest portion of calculus deposited on the lingual surfaces of mandibular incisors. These samples were decontaminated and subjected to gel electrophoresis for DNA extraction. RESULTS: DNA was found in 85% cases. The amount of DNA varied from 21 to 37 μg/ml of dental calculus. CONCLUSION: Dental calculus is a rich reservoir of human DNA. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5887638/ /pubmed/29657492 http://dx.doi.org/10.4103/jfo.jfds_94_16 Text en Copyright: © 2018 Journal of Forensic Dental Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Singh, Udita
Goel, Saurabh
Estimation and quantification of human DNA in dental calculus: A pilot study
title Estimation and quantification of human DNA in dental calculus: A pilot study
title_full Estimation and quantification of human DNA in dental calculus: A pilot study
title_fullStr Estimation and quantification of human DNA in dental calculus: A pilot study
title_full_unstemmed Estimation and quantification of human DNA in dental calculus: A pilot study
title_short Estimation and quantification of human DNA in dental calculus: A pilot study
title_sort estimation and quantification of human dna in dental calculus: a pilot study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887638/
https://www.ncbi.nlm.nih.gov/pubmed/29657492
http://dx.doi.org/10.4103/jfo.jfds_94_16
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