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Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice

OBJECTIVE: The present study was performed to investigate the effect of hydroalcoholic extract of red cabbage and its fractions on sleeping behavior in mice. MATERIALS AND METHODS: The extract and its fractions were injected to mice and sleep duration as well as sleep latency were recorded. Furtherm...

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Autores principales: Hosseini, Azar, Sobhanifar, Mohammad-Ali, Forouzanfar, Fatemeh, Aghaee, Azita, Rakhshandeh, Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887652/
https://www.ncbi.nlm.nih.gov/pubmed/29657508
http://dx.doi.org/10.4103/jpbs.JPBS_215_17
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author Hosseini, Azar
Sobhanifar, Mohammad-Ali
Forouzanfar, Fatemeh
Aghaee, Azita
Rakhshandeh, Hassan
author_facet Hosseini, Azar
Sobhanifar, Mohammad-Ali
Forouzanfar, Fatemeh
Aghaee, Azita
Rakhshandeh, Hassan
author_sort Hosseini, Azar
collection PubMed
description OBJECTIVE: The present study was performed to investigate the effect of hydroalcoholic extract of red cabbage and its fractions on sleeping behavior in mice. MATERIALS AND METHODS: The extract and its fractions were injected to mice and sleep duration as well as sleep latency were recorded. Furthermore, toxicity of the extract was determined both in vivo and in vitro. RESULTS: The extract increased sleep duration at doses of 50–200mg/kg (P < 0.001). This observed hypnotic effect was comparable to that of diazepam (3mg/kg) (P < 0.001 in comparison with control group). Ethyl acetate, n-butanol, and aqueous fractions could increase sleep duration (P < 0.001). The sleep latency was decreased by the extract (P < 0.001) and only ethyl acetate fraction (P < 0.001). LD(50) value for red cabbage extract was 2.4g/kg. There was no toxic effect on viability of cultured neuronal cells (PC12). Rotarod test results showed that there were no significant differences between the extract groups and the control group. CONCLUSION: The results suggest that red cabbage potentiates pentobarbital hypnosis without any toxic effect. The main component(s) responsible for this effect is most likely to be intermediate polar agent(s) such as flavonoids, which are found in ethyl acetate fraction of this plant.
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spelling pubmed-58876522018-04-13 Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice Hosseini, Azar Sobhanifar, Mohammad-Ali Forouzanfar, Fatemeh Aghaee, Azita Rakhshandeh, Hassan J Pharm Bioallied Sci Original Article OBJECTIVE: The present study was performed to investigate the effect of hydroalcoholic extract of red cabbage and its fractions on sleeping behavior in mice. MATERIALS AND METHODS: The extract and its fractions were injected to mice and sleep duration as well as sleep latency were recorded. Furthermore, toxicity of the extract was determined both in vivo and in vitro. RESULTS: The extract increased sleep duration at doses of 50–200mg/kg (P < 0.001). This observed hypnotic effect was comparable to that of diazepam (3mg/kg) (P < 0.001 in comparison with control group). Ethyl acetate, n-butanol, and aqueous fractions could increase sleep duration (P < 0.001). The sleep latency was decreased by the extract (P < 0.001) and only ethyl acetate fraction (P < 0.001). LD(50) value for red cabbage extract was 2.4g/kg. There was no toxic effect on viability of cultured neuronal cells (PC12). Rotarod test results showed that there were no significant differences between the extract groups and the control group. CONCLUSION: The results suggest that red cabbage potentiates pentobarbital hypnosis without any toxic effect. The main component(s) responsible for this effect is most likely to be intermediate polar agent(s) such as flavonoids, which are found in ethyl acetate fraction of this plant. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5887652/ /pubmed/29657508 http://dx.doi.org/10.4103/jpbs.JPBS_215_17 Text en Copyright: © 2018 Journal of Pharmacy And Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Hosseini, Azar
Sobhanifar, Mohammad-Ali
Forouzanfar, Fatemeh
Aghaee, Azita
Rakhshandeh, Hassan
Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice
title Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice
title_full Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice
title_fullStr Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice
title_full_unstemmed Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice
title_short Hypnotic Effect of Red Cabbage (Brassica oleracea) on Pentobarbital-Induced Sleep in Mice
title_sort hypnotic effect of red cabbage (brassica oleracea) on pentobarbital-induced sleep in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887652/
https://www.ncbi.nlm.nih.gov/pubmed/29657508
http://dx.doi.org/10.4103/jpbs.JPBS_215_17
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