Noncanonical substrate preference of lambda exonuclease for 5′-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction

Lambda exonuclease (λ exo) plays an important role in the resection of DNA ends for DNA repair. Currently, it is also a widely used enzymatic tool in genetic engineering, DNA-binding protein mapping, nanopore sequencing and biosensing. Herein, we disclose two noncanonical properties of this enzyme a...

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Detalles Bibliográficos
Autores principales: Wu, Tongbo, Yang, Yufei, Chen, Wei, Wang, Jiayu, Yang, Ziyu, Wang, Shenlin, Xiao, Xianjin, Li, Mengyuan, Zhao, Meiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5888420/
https://www.ncbi.nlm.nih.gov/pubmed/29490081
http://dx.doi.org/10.1093/nar/gky154

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5888420/
https://www.ncbi.nlm.nih.gov/pubmed/29490081
http://dx.doi.org/10.1093/nar/gky154

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