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DNA mismatch repair and oligonucleotide end-protection promote base-pair substitution distal from a CRISPR/Cas9-induced DNA break

Single-stranded oligodeoxyribonucleotide (ssODN)-mediated repair of CRISPR/Cas9-induced DNA double-strand breaks (DSB) can effectively be used to introduce small genomic alterations in a defined locus. Here, we reveal DNA mismatch repair (MMR) activity is crucial for efficient nucleotide substitutio...

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Detalles Bibliográficos
Autores principales:	Harmsen, Tim, Klaasen, Sjoerd, van de Vrugt, Henri, te Riele, Hein
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Genome Integrity, Repair and Replication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5888797/ https://www.ncbi.nlm.nih.gov/pubmed/29447381 http://dx.doi.org/10.1093/nar/gky076

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