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Distinct gibberellin functions during and after grapevine bud dormancy release

The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It has been hypothesized that (i) abscisic acid (ABA) represses bud–meristem activity; (ii) perturbation of respiration induces an interplay between ethylene and ABA metabolism, which leads to removal of repress...

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Autores principales: Zheng, Chuanlin, Kwame Acheampong, Atiako, Shi, Zhaowan, Halaly, Tamar, Kamiya, Yuji, Ophir, Ron, Galbraith, David W, Or, Etti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5888973/
https://www.ncbi.nlm.nih.gov/pubmed/29385616
http://dx.doi.org/10.1093/jxb/ery022
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author Zheng, Chuanlin
Kwame Acheampong, Atiako
Shi, Zhaowan
Halaly, Tamar
Kamiya, Yuji
Ophir, Ron
Galbraith, David W
Or, Etti
author_facet Zheng, Chuanlin
Kwame Acheampong, Atiako
Shi, Zhaowan
Halaly, Tamar
Kamiya, Yuji
Ophir, Ron
Galbraith, David W
Or, Etti
author_sort Zheng, Chuanlin
collection PubMed
description The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It has been hypothesized that (i) abscisic acid (ABA) represses bud–meristem activity; (ii) perturbation of respiration induces an interplay between ethylene and ABA metabolism, which leads to removal of repression; and (iii) gibberellin (GA)-mediated growth is resumed. The first two hypothesis have been formally supported. The current study examines the third hypothesis regarding the potential involvement of GA in dormancy release. We found that during natural dormancy induction, levels of VvGA3ox, VvGA20ox, and VvGASA2 transcripts and of GA(1) were decreased. However, during dormancy release, expression of these genes was enhanced, accompanied by decreased expression of the bud-expressed GA-deactivating VvGA2ox. Despite indications for its positive role during natural dormancy release, GA application had inhibitory effects on bud break. Hydrogen cyanamide up-regulated VvGA2ox and down-regulated VvGA3ox and VvGA20ox expression, reduced GA(1) levels, and partially rescued the negative effect of GA. GA had an inhibitory effect only when applied simultaneously with bud-forcing initiation. Given these results, we hypothesize that during initial activation of the dormant bud meristem, the level of GA must be restricted, but after meristem activation an increase in its level serves to enhance primordia regrowth.
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spelling pubmed-58889732018-11-14 Distinct gibberellin functions during and after grapevine bud dormancy release Zheng, Chuanlin Kwame Acheampong, Atiako Shi, Zhaowan Halaly, Tamar Kamiya, Yuji Ophir, Ron Galbraith, David W Or, Etti J Exp Bot Research Papers The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It has been hypothesized that (i) abscisic acid (ABA) represses bud–meristem activity; (ii) perturbation of respiration induces an interplay between ethylene and ABA metabolism, which leads to removal of repression; and (iii) gibberellin (GA)-mediated growth is resumed. The first two hypothesis have been formally supported. The current study examines the third hypothesis regarding the potential involvement of GA in dormancy release. We found that during natural dormancy induction, levels of VvGA3ox, VvGA20ox, and VvGASA2 transcripts and of GA(1) were decreased. However, during dormancy release, expression of these genes was enhanced, accompanied by decreased expression of the bud-expressed GA-deactivating VvGA2ox. Despite indications for its positive role during natural dormancy release, GA application had inhibitory effects on bud break. Hydrogen cyanamide up-regulated VvGA2ox and down-regulated VvGA3ox and VvGA20ox expression, reduced GA(1) levels, and partially rescued the negative effect of GA. GA had an inhibitory effect only when applied simultaneously with bud-forcing initiation. Given these results, we hypothesize that during initial activation of the dormant bud meristem, the level of GA must be restricted, but after meristem activation an increase in its level serves to enhance primordia regrowth. Oxford University Press 2018-03-16 2018-01-27 /pmc/articles/PMC5888973/ /pubmed/29385616 http://dx.doi.org/10.1093/jxb/ery022 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Zheng, Chuanlin
Kwame Acheampong, Atiako
Shi, Zhaowan
Halaly, Tamar
Kamiya, Yuji
Ophir, Ron
Galbraith, David W
Or, Etti
Distinct gibberellin functions during and after grapevine bud dormancy release
title Distinct gibberellin functions during and after grapevine bud dormancy release
title_full Distinct gibberellin functions during and after grapevine bud dormancy release
title_fullStr Distinct gibberellin functions during and after grapevine bud dormancy release
title_full_unstemmed Distinct gibberellin functions during and after grapevine bud dormancy release
title_short Distinct gibberellin functions during and after grapevine bud dormancy release
title_sort distinct gibberellin functions during and after grapevine bud dormancy release
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5888973/
https://www.ncbi.nlm.nih.gov/pubmed/29385616
http://dx.doi.org/10.1093/jxb/ery022
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