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Quantification of Collagen Organization after Nerve Repair
BACKGROUND: Clinical outcomes after nerve injury and repair remain suboptimal. Patients may be plagued by poor functional recovery and painful neuroma at the repair site, characterized by disorganized collagen and sprouting axons. Collagen deposition during wound healing can be intrinsically imaged...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889458/ https://www.ncbi.nlm.nih.gov/pubmed/29632766 http://dx.doi.org/10.1097/GOX.0000000000001586 |
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author | Israel, Jacqueline S. Esquibel, Corinne R. Dingle, Aaron M. Liu, Yuming Keikhosravi, Adib Pisaniello, Jane A. Hesse, Madison A. Brodnick, Sarah K. Novello, Joseph Krugner-Higby, Lisa Williams, Justin C. Eliceiri, Kevin W. Poore, Samuel O. |
author_facet | Israel, Jacqueline S. Esquibel, Corinne R. Dingle, Aaron M. Liu, Yuming Keikhosravi, Adib Pisaniello, Jane A. Hesse, Madison A. Brodnick, Sarah K. Novello, Joseph Krugner-Higby, Lisa Williams, Justin C. Eliceiri, Kevin W. Poore, Samuel O. |
author_sort | Israel, Jacqueline S. |
collection | PubMed |
description | BACKGROUND: Clinical outcomes after nerve injury and repair remain suboptimal. Patients may be plagued by poor functional recovery and painful neuroma at the repair site, characterized by disorganized collagen and sprouting axons. Collagen deposition during wound healing can be intrinsically imaged using second harmonic generation (SHG) microscopy. The purpose of this study was to develop a protocol for SHG imaging of nerves and to assess whether collagen alignment can be quantified after nerve repair. METHODS: Sciatic nerve transection and epineural repair was performed in male rats. The contralateral nerves were used as intra-animal controls. Ten-millimeter nerve segments were harvested and fixed onto slides. SHG images were collected using a 20× objective on a multiphoton microscope. Collagen fiber alignment was calculated using CurveAlign software. Alignment was calculated on a scale from 0 to 1, where 1 represents perfect alignment. Statistical analysis was performed using a linear mixed-effects model. RESULTS: Eight male rats underwent right sciatic nerve repair using 9-0 Nylon suture. There were gross variations in collagen fiber organization in the repaired nerves compared with the controls. Quantitatively, collagen fibers were more aligned in the control nerves (mean alignment 0.754, SE 0.055) than in the repairs (mean alignment 0.413, SE 0.047; P < 0.001). CONCLUSIONS: SHG microscopy can be used to quantitate collagen after nerve repair via fiber alignment. Given that the development of neuroma likely reflects aberrant wound healing, ex vivo and/or in vivo SHG imaging may be useful for further investigation of the variables predisposing to neuroma. |
format | Online Article Text |
id | pubmed-5889458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-58894582018-04-09 Quantification of Collagen Organization after Nerve Repair Israel, Jacqueline S. Esquibel, Corinne R. Dingle, Aaron M. Liu, Yuming Keikhosravi, Adib Pisaniello, Jane A. Hesse, Madison A. Brodnick, Sarah K. Novello, Joseph Krugner-Higby, Lisa Williams, Justin C. Eliceiri, Kevin W. Poore, Samuel O. Plast Reconstr Surg Glob Open Experimental BACKGROUND: Clinical outcomes after nerve injury and repair remain suboptimal. Patients may be plagued by poor functional recovery and painful neuroma at the repair site, characterized by disorganized collagen and sprouting axons. Collagen deposition during wound healing can be intrinsically imaged using second harmonic generation (SHG) microscopy. The purpose of this study was to develop a protocol for SHG imaging of nerves and to assess whether collagen alignment can be quantified after nerve repair. METHODS: Sciatic nerve transection and epineural repair was performed in male rats. The contralateral nerves were used as intra-animal controls. Ten-millimeter nerve segments were harvested and fixed onto slides. SHG images were collected using a 20× objective on a multiphoton microscope. Collagen fiber alignment was calculated using CurveAlign software. Alignment was calculated on a scale from 0 to 1, where 1 represents perfect alignment. Statistical analysis was performed using a linear mixed-effects model. RESULTS: Eight male rats underwent right sciatic nerve repair using 9-0 Nylon suture. There were gross variations in collagen fiber organization in the repaired nerves compared with the controls. Quantitatively, collagen fibers were more aligned in the control nerves (mean alignment 0.754, SE 0.055) than in the repairs (mean alignment 0.413, SE 0.047; P < 0.001). CONCLUSIONS: SHG microscopy can be used to quantitate collagen after nerve repair via fiber alignment. Given that the development of neuroma likely reflects aberrant wound healing, ex vivo and/or in vivo SHG imaging may be useful for further investigation of the variables predisposing to neuroma. Wolters Kluwer Health 2017-12-11 /pmc/articles/PMC5889458/ /pubmed/29632766 http://dx.doi.org/10.1097/GOX.0000000000001586 Text en Copyright © 2017 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Experimental Israel, Jacqueline S. Esquibel, Corinne R. Dingle, Aaron M. Liu, Yuming Keikhosravi, Adib Pisaniello, Jane A. Hesse, Madison A. Brodnick, Sarah K. Novello, Joseph Krugner-Higby, Lisa Williams, Justin C. Eliceiri, Kevin W. Poore, Samuel O. Quantification of Collagen Organization after Nerve Repair |
title | Quantification of Collagen Organization after Nerve Repair |
title_full | Quantification of Collagen Organization after Nerve Repair |
title_fullStr | Quantification of Collagen Organization after Nerve Repair |
title_full_unstemmed | Quantification of Collagen Organization after Nerve Repair |
title_short | Quantification of Collagen Organization after Nerve Repair |
title_sort | quantification of collagen organization after nerve repair |
topic | Experimental |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889458/ https://www.ncbi.nlm.nih.gov/pubmed/29632766 http://dx.doi.org/10.1097/GOX.0000000000001586 |
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