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Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal

The folding and insertion of integral β-barrel membrane proteins into the outer membrane of Gram-negative bacteria is required for viability and bacterial pathogenesis. Unfortunately, the lack of selective and potent modulators to dissect β-barrel folding in vivo has hampered our understanding of th...

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Autores principales: Storek, Kelly M., Auerbach, Marcy R., Shi, Handuo, Garcia, Natalie K., Sun, Dawei, Nickerson, Nicholas N., Vij, Rajesh, Lin, Zhonghua, Chiang, Nancy, Schneider, Kellen, Wecksler, Aaron T., Skippington, Elizabeth, Nakamura, Gerald, Seshasayee, Dhaya, Koerber, James T., Payandeh, Jian, Smith, Peter A., Rutherford, Steven T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889671/
https://www.ncbi.nlm.nih.gov/pubmed/29555747
http://dx.doi.org/10.1073/pnas.1800043115
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author Storek, Kelly M.
Auerbach, Marcy R.
Shi, Handuo
Garcia, Natalie K.
Sun, Dawei
Nickerson, Nicholas N.
Vij, Rajesh
Lin, Zhonghua
Chiang, Nancy
Schneider, Kellen
Wecksler, Aaron T.
Skippington, Elizabeth
Nakamura, Gerald
Seshasayee, Dhaya
Koerber, James T.
Payandeh, Jian
Smith, Peter A.
Rutherford, Steven T.
author_facet Storek, Kelly M.
Auerbach, Marcy R.
Shi, Handuo
Garcia, Natalie K.
Sun, Dawei
Nickerson, Nicholas N.
Vij, Rajesh
Lin, Zhonghua
Chiang, Nancy
Schneider, Kellen
Wecksler, Aaron T.
Skippington, Elizabeth
Nakamura, Gerald
Seshasayee, Dhaya
Koerber, James T.
Payandeh, Jian
Smith, Peter A.
Rutherford, Steven T.
author_sort Storek, Kelly M.
collection PubMed
description The folding and insertion of integral β-barrel membrane proteins into the outer membrane of Gram-negative bacteria is required for viability and bacterial pathogenesis. Unfortunately, the lack of selective and potent modulators to dissect β-barrel folding in vivo has hampered our understanding of this fundamental biological process. Here, we characterize a monoclonal antibody that selectively inhibits an essential component of the Escherichia coli β-barrel assembly machine, BamA. In the absence of complement or other immune factors, the unmodified antibody MAB1 demonstrates bactericidal activity against an E. coli strain with truncated LPS. Direct binding of MAB1 to an extracellular BamA epitope inhibits its β-barrel folding activity, induces periplasmic stress, disrupts outer membrane integrity, and kills bacteria. Notably, resistance to MAB1-mediated killing reveals a link between outer membrane fluidity and protein folding by BamA in vivo, underscoring the utility of this antibody for studying β-barrel membrane protein folding within a living cell. Identification of this BamA antagonist highlights the potential for new mechanisms of antibiotics to inhibit Gram-negative bacterial growth by targeting extracellular epitopes.
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spelling pubmed-58896712018-04-09 Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal Storek, Kelly M. Auerbach, Marcy R. Shi, Handuo Garcia, Natalie K. Sun, Dawei Nickerson, Nicholas N. Vij, Rajesh Lin, Zhonghua Chiang, Nancy Schneider, Kellen Wecksler, Aaron T. Skippington, Elizabeth Nakamura, Gerald Seshasayee, Dhaya Koerber, James T. Payandeh, Jian Smith, Peter A. Rutherford, Steven T. Proc Natl Acad Sci U S A Biological Sciences The folding and insertion of integral β-barrel membrane proteins into the outer membrane of Gram-negative bacteria is required for viability and bacterial pathogenesis. Unfortunately, the lack of selective and potent modulators to dissect β-barrel folding in vivo has hampered our understanding of this fundamental biological process. Here, we characterize a monoclonal antibody that selectively inhibits an essential component of the Escherichia coli β-barrel assembly machine, BamA. In the absence of complement or other immune factors, the unmodified antibody MAB1 demonstrates bactericidal activity against an E. coli strain with truncated LPS. Direct binding of MAB1 to an extracellular BamA epitope inhibits its β-barrel folding activity, induces periplasmic stress, disrupts outer membrane integrity, and kills bacteria. Notably, resistance to MAB1-mediated killing reveals a link between outer membrane fluidity and protein folding by BamA in vivo, underscoring the utility of this antibody for studying β-barrel membrane protein folding within a living cell. Identification of this BamA antagonist highlights the potential for new mechanisms of antibiotics to inhibit Gram-negative bacterial growth by targeting extracellular epitopes. National Academy of Sciences 2018-04-03 2018-03-19 /pmc/articles/PMC5889671/ /pubmed/29555747 http://dx.doi.org/10.1073/pnas.1800043115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Storek, Kelly M.
Auerbach, Marcy R.
Shi, Handuo
Garcia, Natalie K.
Sun, Dawei
Nickerson, Nicholas N.
Vij, Rajesh
Lin, Zhonghua
Chiang, Nancy
Schneider, Kellen
Wecksler, Aaron T.
Skippington, Elizabeth
Nakamura, Gerald
Seshasayee, Dhaya
Koerber, James T.
Payandeh, Jian
Smith, Peter A.
Rutherford, Steven T.
Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal
title Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal
title_full Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal
title_fullStr Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal
title_full_unstemmed Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal
title_short Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal
title_sort monoclonal antibody targeting the β-barrel assembly machine of escherichia coli is bactericidal
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889671/
https://www.ncbi.nlm.nih.gov/pubmed/29555747
http://dx.doi.org/10.1073/pnas.1800043115
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