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Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)

Streptocarpus teitensis (Gesneriaceae) is an endemic species listed as critically endangered in the International Union for Conservation of Nature (IUCN) red list of threatened species. However, the sequence and genome information of this species remains to be limited. In this article, we present th...

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Autores principales: Kyalo, Cornelius M., Gichira, Andrew W., Li, Zhi-Zhong, Saina, Josphat K., Malombe, Itambo, Hu, Guang-Wan, Wang, Qing-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889905/
https://www.ncbi.nlm.nih.gov/pubmed/29770326
http://dx.doi.org/10.1155/2018/1507847
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author Kyalo, Cornelius M.
Gichira, Andrew W.
Li, Zhi-Zhong
Saina, Josphat K.
Malombe, Itambo
Hu, Guang-Wan
Wang, Qing-Feng
author_facet Kyalo, Cornelius M.
Gichira, Andrew W.
Li, Zhi-Zhong
Saina, Josphat K.
Malombe, Itambo
Hu, Guang-Wan
Wang, Qing-Feng
author_sort Kyalo, Cornelius M.
collection PubMed
description Streptocarpus teitensis (Gesneriaceae) is an endemic species listed as critically endangered in the International Union for Conservation of Nature (IUCN) red list of threatened species. However, the sequence and genome information of this species remains to be limited. In this article, we present the complete chloroplast genome structure of Streptocarpus teitensis and its evolution inferred through comparative studies with other related species. S. teitensis displayed a chloroplast genome size of 153,207 bp, sheltering a pair of inverted repeats (IR) of 25,402 bp each split by small and large single-copy (SSC and LSC) regions of 18,300 and 84,103 bp, respectively. The chloroplast genome was observed to contain 116 unique genes, of which 80 are protein-coding, 32 are transfer RNAs, and four are ribosomal RNAs. In addition, a total of 196 SSR markers were detected in the chloroplast genome of Streptocarpus teitensis with mononucleotides (57.1%) being the majority, followed by trinucleotides (33.2%) and dinucleotides and tetranucleotides (both 4.1%), and pentanucleotides being the least (1.5%). Genome alignment indicated that this genome was comparable to other sequenced members of order Lamiales. The phylogenetic analysis suggested that Streptocarpus teitensis is closely related to Lysionotus pauciflorus and Dorcoceras hygrometricum.
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spelling pubmed-58899052018-05-16 Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae) Kyalo, Cornelius M. Gichira, Andrew W. Li, Zhi-Zhong Saina, Josphat K. Malombe, Itambo Hu, Guang-Wan Wang, Qing-Feng Biomed Res Int Research Article Streptocarpus teitensis (Gesneriaceae) is an endemic species listed as critically endangered in the International Union for Conservation of Nature (IUCN) red list of threatened species. However, the sequence and genome information of this species remains to be limited. In this article, we present the complete chloroplast genome structure of Streptocarpus teitensis and its evolution inferred through comparative studies with other related species. S. teitensis displayed a chloroplast genome size of 153,207 bp, sheltering a pair of inverted repeats (IR) of 25,402 bp each split by small and large single-copy (SSC and LSC) regions of 18,300 and 84,103 bp, respectively. The chloroplast genome was observed to contain 116 unique genes, of which 80 are protein-coding, 32 are transfer RNAs, and four are ribosomal RNAs. In addition, a total of 196 SSR markers were detected in the chloroplast genome of Streptocarpus teitensis with mononucleotides (57.1%) being the majority, followed by trinucleotides (33.2%) and dinucleotides and tetranucleotides (both 4.1%), and pentanucleotides being the least (1.5%). Genome alignment indicated that this genome was comparable to other sequenced members of order Lamiales. The phylogenetic analysis suggested that Streptocarpus teitensis is closely related to Lysionotus pauciflorus and Dorcoceras hygrometricum. Hindawi 2018-03-25 /pmc/articles/PMC5889905/ /pubmed/29770326 http://dx.doi.org/10.1155/2018/1507847 Text en Copyright © 2018 Cornelius M. Kyalo et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kyalo, Cornelius M.
Gichira, Andrew W.
Li, Zhi-Zhong
Saina, Josphat K.
Malombe, Itambo
Hu, Guang-Wan
Wang, Qing-Feng
Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)
title Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)
title_full Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)
title_fullStr Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)
title_full_unstemmed Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)
title_short Characterization and Comparative Analysis of the Complete Chloroplast Genome of the Critically Endangered Species Streptocarpus teitensis (Gesneriaceae)
title_sort characterization and comparative analysis of the complete chloroplast genome of the critically endangered species streptocarpus teitensis (gesneriaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889905/
https://www.ncbi.nlm.nih.gov/pubmed/29770326
http://dx.doi.org/10.1155/2018/1507847
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