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Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion

Smell of very low dose of chemical might evoke subjective physical symptoms in human by some process of learning named the aversion conditioning. But few scientific evidences of the hypothesis have been reported so far. Validity of conditioned odor aversion (COA) using low-doses of organic solvent a...

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Autores principales: HOJO, Rieko, TAKAYA, Mitsutoshi, YASUDA, Akinori, TSUCHIYA, Masao, OGAWA, Yasutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Occupational Safety and Health, Japan 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889932/
https://www.ncbi.nlm.nih.gov/pubmed/29176267
http://dx.doi.org/10.2486/indhealth.2017-0041
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author HOJO, Rieko
TAKAYA, Mitsutoshi
YASUDA, Akinori
TSUCHIYA, Masao
OGAWA, Yasutaka
author_facet HOJO, Rieko
TAKAYA, Mitsutoshi
YASUDA, Akinori
TSUCHIYA, Masao
OGAWA, Yasutaka
author_sort HOJO, Rieko
collection PubMed
description Smell of very low dose of chemical might evoke subjective physical symptoms in human by some process of learning named the aversion conditioning. But few scientific evidences of the hypothesis have been reported so far. Validity of conditioned odor aversion (COA) using low-doses of organic solvent as odor conditioned stimulus (CS) was examined. In conditioning phase, water-deprived male Sprague-Dawley rats were presented low, medium or high dose solution for 30 min followed by 0.3 M Lithium Chloride (LiCl) solution or saline injection. The xylene solution and drink water were simultaneously provided on the next day as two-bottle test. Consumption of medium dose of xylene solution was significantly decreased in LiCl injection group as compared with saline group. There was no difference between LiCl and saline injected animals in low group. Animals in high dose did not access to xylene even on the conditioning. These results indicate that animals showed high sensitivity for discrimination against concentration of xylene and that the medium dose of xylene functioned as the CS. We concluded that the COA used in the present study may be one of useful procedures to investigate olfaction of animal.
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spelling pubmed-58899322018-04-11 Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion HOJO, Rieko TAKAYA, Mitsutoshi YASUDA, Akinori TSUCHIYA, Masao OGAWA, Yasutaka Ind Health Original Article Smell of very low dose of chemical might evoke subjective physical symptoms in human by some process of learning named the aversion conditioning. But few scientific evidences of the hypothesis have been reported so far. Validity of conditioned odor aversion (COA) using low-doses of organic solvent as odor conditioned stimulus (CS) was examined. In conditioning phase, water-deprived male Sprague-Dawley rats were presented low, medium or high dose solution for 30 min followed by 0.3 M Lithium Chloride (LiCl) solution or saline injection. The xylene solution and drink water were simultaneously provided on the next day as two-bottle test. Consumption of medium dose of xylene solution was significantly decreased in LiCl injection group as compared with saline group. There was no difference between LiCl and saline injected animals in low group. Animals in high dose did not access to xylene even on the conditioning. These results indicate that animals showed high sensitivity for discrimination against concentration of xylene and that the medium dose of xylene functioned as the CS. We concluded that the COA used in the present study may be one of useful procedures to investigate olfaction of animal. National Institute of Occupational Safety and Health, Japan 2017-11-23 2018-03 /pmc/articles/PMC5889932/ /pubmed/29176267 http://dx.doi.org/10.2486/indhealth.2017-0041 Text en ©2018 National Institute of Occupational Safety and Health http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
HOJO, Rieko
TAKAYA, Mitsutoshi
YASUDA, Akinori
TSUCHIYA, Masao
OGAWA, Yasutaka
Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
title Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
title_full Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
title_fullStr Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
title_full_unstemmed Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
title_short Examination of validity of a conditioned odor aversion (COA) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
title_sort examination of validity of a conditioned odor aversion (coa) procedure using low-dose of organic solvent as an applied procedure of the conditioned taste aversion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889932/
https://www.ncbi.nlm.nih.gov/pubmed/29176267
http://dx.doi.org/10.2486/indhealth.2017-0041
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