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Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways

Salinity stress is one of the serious factors, limiting production of major agricultural crops; especially, in sodic soils. A number of approaches are being applied to mitigate the salt-induced adverse effects in agricultural crops through implying different halotolerant microbes. In this aspect, a...

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Autores principales: Patel, Vikas K., Srivastava, Ruchi, Sharma, Anjney, Srivastava, Anchal K., Singh, Savita, Srivastava, Alok K., Kashyap, Prem L., Chakdar, Hillol, Pandiyan, K., Kalra, Alok, Saxena, Anil K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890156/
https://www.ncbi.nlm.nih.gov/pubmed/29662469
http://dx.doi.org/10.3389/fmicb.2018.00423
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author Patel, Vikas K.
Srivastava, Ruchi
Sharma, Anjney
Srivastava, Anchal K.
Singh, Savita
Srivastava, Alok K.
Kashyap, Prem L.
Chakdar, Hillol
Pandiyan, K.
Kalra, Alok
Saxena, Anil K.
author_facet Patel, Vikas K.
Srivastava, Ruchi
Sharma, Anjney
Srivastava, Anchal K.
Singh, Savita
Srivastava, Alok K.
Kashyap, Prem L.
Chakdar, Hillol
Pandiyan, K.
Kalra, Alok
Saxena, Anil K.
author_sort Patel, Vikas K.
collection PubMed
description Salinity stress is one of the serious factors, limiting production of major agricultural crops; especially, in sodic soils. A number of approaches are being applied to mitigate the salt-induced adverse effects in agricultural crops through implying different halotolerant microbes. In this aspect, a halotolerant, Exiguobacterium profundum PHM11 was evaluated under eight different salinity regimes; 100, 250, 500, 1000, 1500, 2000, 2500, and 3000 mM to know its inherent salt tolerance limits and salt-induced consequences affecting its natural metabolism. Based on the stoichiometric growth kinetics; 100 and 1500 mM concentrations were selected as optimal and minimal performance limits for PHM11. To know, how salt stress affects the expression profiles of regulatory genes of its key metabolic pathways, and total production of important metabolites; biomass, carotenoids, beta-carotene production, IAA and proline contents, and expression profiles of key genes affecting the protein folding, structural adaptations, transportation across the cell membrane, stress tolerance, carotenoids, IAA and mannitol production in PHM11 were studied under 100 and 1500 mM salinity. E. profundum PHM11 showed maximum and minimum growth, biomass and metabolite production at 100 and 1500 mM salinity respectively. Salt-induced fine-tuning of expression profiles of key genes of stress pathways was determined in halotolerant bacterium PHM11.
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spelling pubmed-58901562018-04-16 Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways Patel, Vikas K. Srivastava, Ruchi Sharma, Anjney Srivastava, Anchal K. Singh, Savita Srivastava, Alok K. Kashyap, Prem L. Chakdar, Hillol Pandiyan, K. Kalra, Alok Saxena, Anil K. Front Microbiol Microbiology Salinity stress is one of the serious factors, limiting production of major agricultural crops; especially, in sodic soils. A number of approaches are being applied to mitigate the salt-induced adverse effects in agricultural crops through implying different halotolerant microbes. In this aspect, a halotolerant, Exiguobacterium profundum PHM11 was evaluated under eight different salinity regimes; 100, 250, 500, 1000, 1500, 2000, 2500, and 3000 mM to know its inherent salt tolerance limits and salt-induced consequences affecting its natural metabolism. Based on the stoichiometric growth kinetics; 100 and 1500 mM concentrations were selected as optimal and minimal performance limits for PHM11. To know, how salt stress affects the expression profiles of regulatory genes of its key metabolic pathways, and total production of important metabolites; biomass, carotenoids, beta-carotene production, IAA and proline contents, and expression profiles of key genes affecting the protein folding, structural adaptations, transportation across the cell membrane, stress tolerance, carotenoids, IAA and mannitol production in PHM11 were studied under 100 and 1500 mM salinity. E. profundum PHM11 showed maximum and minimum growth, biomass and metabolite production at 100 and 1500 mM salinity respectively. Salt-induced fine-tuning of expression profiles of key genes of stress pathways was determined in halotolerant bacterium PHM11. Frontiers Media S.A. 2018-03-12 /pmc/articles/PMC5890156/ /pubmed/29662469 http://dx.doi.org/10.3389/fmicb.2018.00423 Text en Copyright © 2018 Patel, Srivastava, Sharma, Srivastava, Singh, Srivastava, Kashyap, Chakdar, Pandiyan, Kalra and Saxena. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Patel, Vikas K.
Srivastava, Ruchi
Sharma, Anjney
Srivastava, Anchal K.
Singh, Savita
Srivastava, Alok K.
Kashyap, Prem L.
Chakdar, Hillol
Pandiyan, K.
Kalra, Alok
Saxena, Anil K.
Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
title Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
title_full Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
title_fullStr Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
title_full_unstemmed Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
title_short Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
title_sort halotolerant exiguobacterium profundum phm11 tolerate salinity by accumulating l-proline and fine-tuning gene expression profiles of related metabolic pathways
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890156/
https://www.ncbi.nlm.nih.gov/pubmed/29662469
http://dx.doi.org/10.3389/fmicb.2018.00423
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