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The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae

Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA...

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Autores principales: Li, Huan, Liu, Feng, Peng, Wei, Yan, Kang, Zhao, Haixu, Liu, Ting, Cheng, Hui, Chang, Peixi, Yuan, Fangyan, Chen, Huanchun, Bei, Weicheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890194/
https://www.ncbi.nlm.nih.gov/pubmed/29662838
http://dx.doi.org/10.3389/fcimb.2018.00072
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author Li, Huan
Liu, Feng
Peng, Wei
Yan, Kang
Zhao, Haixu
Liu, Ting
Cheng, Hui
Chang, Peixi
Yuan, Fangyan
Chen, Huanchun
Bei, Weicheng
author_facet Li, Huan
Liu, Feng
Peng, Wei
Yan, Kang
Zhao, Haixu
Liu, Ting
Cheng, Hui
Chang, Peixi
Yuan, Fangyan
Chen, Huanchun
Bei, Weicheng
author_sort Li, Huan
collection PubMed
description Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae.
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spelling pubmed-58901942018-04-16 The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae Li, Huan Liu, Feng Peng, Wei Yan, Kang Zhao, Haixu Liu, Ting Cheng, Hui Chang, Peixi Yuan, Fangyan Chen, Huanchun Bei, Weicheng Front Cell Infect Microbiol Microbiology Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae. Frontiers Media S.A. 2018-03-20 /pmc/articles/PMC5890194/ /pubmed/29662838 http://dx.doi.org/10.3389/fcimb.2018.00072 Text en Copyright © 2018 Li, Liu, Peng, Yan, Zhao, Liu, Cheng, Chang, Yuan, Chen and Bei. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Huan
Liu, Feng
Peng, Wei
Yan, Kang
Zhao, Haixu
Liu, Ting
Cheng, Hui
Chang, Peixi
Yuan, Fangyan
Chen, Huanchun
Bei, Weicheng
The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
title The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
title_full The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
title_fullStr The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
title_full_unstemmed The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
title_short The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
title_sort cpxa/cpxr two-component system affects biofilm formation and virulence in actinobacillus pleuropneumoniae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890194/
https://www.ncbi.nlm.nih.gov/pubmed/29662838
http://dx.doi.org/10.3389/fcimb.2018.00072
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