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Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
Several species of the Gram-negative genus Bordetella are the cause of respiratory infections in mammals and birds, including whooping cough (pertussis) in humans. Very recently, a novel atypical species, Bordetella pseudohinzii, was isolated from laboratory mice. These mice presented no obvious cli...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890243/ https://www.ncbi.nlm.nih.gov/pubmed/29632402 http://dx.doi.org/10.1038/s41598-018-23830-4 |
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author | Perniss, Alexander Schmidt, Nadine Gurtner, Corinne Dietert, Kristina Schwengers, Oliver Weigel, Markus Hempe, Julia Ewers, Christa Pfeil, Uwe Gärtner, Ulrich Gruber, Achim D. Hain, Torsten Kummer, Wolfgang |
author_facet | Perniss, Alexander Schmidt, Nadine Gurtner, Corinne Dietert, Kristina Schwengers, Oliver Weigel, Markus Hempe, Julia Ewers, Christa Pfeil, Uwe Gärtner, Ulrich Gruber, Achim D. Hain, Torsten Kummer, Wolfgang |
author_sort | Perniss, Alexander |
collection | PubMed |
description | Several species of the Gram-negative genus Bordetella are the cause of respiratory infections in mammals and birds, including whooping cough (pertussis) in humans. Very recently, a novel atypical species, Bordetella pseudohinzii, was isolated from laboratory mice. These mice presented no obvious clinical symptoms but elevated numbers of neutrophils in bronchoalveolar lavage fluid and inflammatory signs in histopathology. We noted that this species can occur at high prevalence in a mouse facility despite regular pathogen testing according to the FELASA-recommendations. Affected C57BL/6 J mice had, in addition to the reported pulmonary alterations, tracheal inflammation with reduced numbers of ciliated cells, slower ciliary beat frequency, and largely (>50%) compromised cilia-driven particle transport speed on the mucosal surface, a primary innate defence mechanism. In an in vitro-model, Bordetella pseudohinzii attached to respiratory kinocilia, impaired ciliary function within 4 h and caused epithelial damage within 24 h. Regular testing for this ciliotropic Bordetella species and excluding it from colonies that provide mice for lung research shall be recommended. On the other hand, controlled colonization and infection with Bordetella pseudohinzii may serve as an experimental model to investigate mechanisms of mucociliary clearance and microbial strategies to escape from this primary innate defence response. |
format | Online Article Text |
id | pubmed-5890243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58902432018-04-13 Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Perniss, Alexander Schmidt, Nadine Gurtner, Corinne Dietert, Kristina Schwengers, Oliver Weigel, Markus Hempe, Julia Ewers, Christa Pfeil, Uwe Gärtner, Ulrich Gruber, Achim D. Hain, Torsten Kummer, Wolfgang Sci Rep Article Several species of the Gram-negative genus Bordetella are the cause of respiratory infections in mammals and birds, including whooping cough (pertussis) in humans. Very recently, a novel atypical species, Bordetella pseudohinzii, was isolated from laboratory mice. These mice presented no obvious clinical symptoms but elevated numbers of neutrophils in bronchoalveolar lavage fluid and inflammatory signs in histopathology. We noted that this species can occur at high prevalence in a mouse facility despite regular pathogen testing according to the FELASA-recommendations. Affected C57BL/6 J mice had, in addition to the reported pulmonary alterations, tracheal inflammation with reduced numbers of ciliated cells, slower ciliary beat frequency, and largely (>50%) compromised cilia-driven particle transport speed on the mucosal surface, a primary innate defence mechanism. In an in vitro-model, Bordetella pseudohinzii attached to respiratory kinocilia, impaired ciliary function within 4 h and caused epithelial damage within 24 h. Regular testing for this ciliotropic Bordetella species and excluding it from colonies that provide mice for lung research shall be recommended. On the other hand, controlled colonization and infection with Bordetella pseudohinzii may serve as an experimental model to investigate mechanisms of mucociliary clearance and microbial strategies to escape from this primary innate defence response. Nature Publishing Group UK 2018-04-09 /pmc/articles/PMC5890243/ /pubmed/29632402 http://dx.doi.org/10.1038/s41598-018-23830-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Perniss, Alexander Schmidt, Nadine Gurtner, Corinne Dietert, Kristina Schwengers, Oliver Weigel, Markus Hempe, Julia Ewers, Christa Pfeil, Uwe Gärtner, Ulrich Gruber, Achim D. Hain, Torsten Kummer, Wolfgang Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
title | Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
title_full | Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
title_fullStr | Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
title_full_unstemmed | Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
title_short | Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
title_sort | bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890243/ https://www.ncbi.nlm.nih.gov/pubmed/29632402 http://dx.doi.org/10.1038/s41598-018-23830-4 |
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