Cargando…
Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer
The cell cycle‐related and expression‐elevated protein in tumor (CREPT) is overexpressed in several human malignancies. However, the clinical relevance of CREPT expression and its biological role in non‐small‐cell lung cancer (NSCLC) remains unclear. In this study, we detected the expression of CREP...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891170/ https://www.ncbi.nlm.nih.gov/pubmed/29397041 http://dx.doi.org/10.1111/cas.13524 |
_version_ | 1783312969932734464 |
---|---|
author | Li, Weimiao Zheng, Guoxu Xia, Jinghua Yang, Guang Sun, Jianyong Wang, Xuejiao Wen, Miaomiao Sun, Ying Zhang, Zhipei Jin, Faguang |
author_facet | Li, Weimiao Zheng, Guoxu Xia, Jinghua Yang, Guang Sun, Jianyong Wang, Xuejiao Wen, Miaomiao Sun, Ying Zhang, Zhipei Jin, Faguang |
author_sort | Li, Weimiao |
collection | PubMed |
description | The cell cycle‐related and expression‐elevated protein in tumor (CREPT) is overexpressed in several human malignancies. However, the clinical relevance of CREPT expression and its biological role in non‐small‐cell lung cancer (NSCLC) remains unclear. In this study, we detected the expression of CREPT in both NSCLC tissues and cell lines by immunohistochemistry, Western blot analysis, and RT‐PCR. The correlation between CREPT expression and clinicopathologic features was analyzed in 271 NSCLC patients. The prognostic value of CREPT expression was evaluated by Kaplan–Meier analysis and Cox regression analysis. CREPT was overexpressed in Calu‐1 cell lines by using plasmid vector and its biological function was explored both in vitro and in vivo. We found that CREPT was significantly overexpressed in NSCLC compared with paired adjacent non‐tumor tissues, and the expression level of CREPT was correlated with tumor differentiation, lymph node metastasis, and clinical stage. Kaplan–Meier analysis showed that the recurrence‐free survival and overall survival of high CREPT expression groups were significantly shorter than those of the low CREPT expression group. Multivariate analysis identified that CREPT might be an independent biomarker for the prediction of NSCLC prognosis. Overexpression of CREPT increased cell proliferation and enhanced the migration and invasion ability of Calu‐1 cells (a human NSCLC cell line with relative low CRPET expression) in vitro. Moreover, CREPT overexpression promoted tumor growth in a nude mice model. These results suggest that CREPT is closely relevant to the proliferation of NSCLC cells and it might be a potential prognostic marker in NSCLC patients. |
format | Online Article Text |
id | pubmed-5891170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58911702018-04-13 Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer Li, Weimiao Zheng, Guoxu Xia, Jinghua Yang, Guang Sun, Jianyong Wang, Xuejiao Wen, Miaomiao Sun, Ying Zhang, Zhipei Jin, Faguang Cancer Sci Original Articles The cell cycle‐related and expression‐elevated protein in tumor (CREPT) is overexpressed in several human malignancies. However, the clinical relevance of CREPT expression and its biological role in non‐small‐cell lung cancer (NSCLC) remains unclear. In this study, we detected the expression of CREPT in both NSCLC tissues and cell lines by immunohistochemistry, Western blot analysis, and RT‐PCR. The correlation between CREPT expression and clinicopathologic features was analyzed in 271 NSCLC patients. The prognostic value of CREPT expression was evaluated by Kaplan–Meier analysis and Cox regression analysis. CREPT was overexpressed in Calu‐1 cell lines by using plasmid vector and its biological function was explored both in vitro and in vivo. We found that CREPT was significantly overexpressed in NSCLC compared with paired adjacent non‐tumor tissues, and the expression level of CREPT was correlated with tumor differentiation, lymph node metastasis, and clinical stage. Kaplan–Meier analysis showed that the recurrence‐free survival and overall survival of high CREPT expression groups were significantly shorter than those of the low CREPT expression group. Multivariate analysis identified that CREPT might be an independent biomarker for the prediction of NSCLC prognosis. Overexpression of CREPT increased cell proliferation and enhanced the migration and invasion ability of Calu‐1 cells (a human NSCLC cell line with relative low CRPET expression) in vitro. Moreover, CREPT overexpression promoted tumor growth in a nude mice model. These results suggest that CREPT is closely relevant to the proliferation of NSCLC cells and it might be a potential prognostic marker in NSCLC patients. John Wiley and Sons Inc. 2018-03-05 2018-04 /pmc/articles/PMC5891170/ /pubmed/29397041 http://dx.doi.org/10.1111/cas.13524 Text en © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Li, Weimiao Zheng, Guoxu Xia, Jinghua Yang, Guang Sun, Jianyong Wang, Xuejiao Wen, Miaomiao Sun, Ying Zhang, Zhipei Jin, Faguang Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
title | Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
title_full | Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
title_fullStr | Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
title_full_unstemmed | Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
title_short | Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
title_sort | cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891170/ https://www.ncbi.nlm.nih.gov/pubmed/29397041 http://dx.doi.org/10.1111/cas.13524 |
work_keys_str_mv | AT liweimiao cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT zhengguoxu cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT xiajinghua cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT yangguang cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT sunjianyong cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT wangxuejiao cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT wenmiaomiao cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT sunying cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT zhangzhipei cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer AT jinfaguang cellcyclerelatedandexpressionelevatedproteinintumoroverexpressionisassociatedwithproliferationbehaviorsandpoorprognosisinnonsmallcelllungcancer |