Cargando…

X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex

The linker of nucleoskeleton and cytoskeleton (LINC) complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, including nuclear migration, mechanotransduction, chromatin tethering and DNA damage response. We recently showed that a nuclear envelope p...

Descripción completa

Detalles Bibliográficos
Autores principales: Imaizumi, Hiromasa, Sato, Katsutoshi, Nishihara, Asuka, Minami, Kazumasa, Koizumi, Masahiko, Matsuura, Nariaki, Hieda, Miki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891189/
https://www.ncbi.nlm.nih.gov/pubmed/29465769
http://dx.doi.org/10.1111/cas.13545
_version_ 1783312974171078656
author Imaizumi, Hiromasa
Sato, Katsutoshi
Nishihara, Asuka
Minami, Kazumasa
Koizumi, Masahiko
Matsuura, Nariaki
Hieda, Miki
author_facet Imaizumi, Hiromasa
Sato, Katsutoshi
Nishihara, Asuka
Minami, Kazumasa
Koizumi, Masahiko
Matsuura, Nariaki
Hieda, Miki
author_sort Imaizumi, Hiromasa
collection PubMed
description The linker of nucleoskeleton and cytoskeleton (LINC) complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, including nuclear migration, mechanotransduction, chromatin tethering and DNA damage response. We recently showed that a nuclear envelope protein, Sad1 and UNC84 domain protein 1 (SUN1), a component of the LINC complex, has a critical function in cell migration. Although ionizing radiation activates cell migration and invasion in vivo and in vitro, the underlying molecular mechanism remains unknown. Here, we examined the involvement of the LINC complex in radiation‐enhanced cell migration and invasion. A sublethal dose of X‐ray radiation promoted human breast cancer MDA‐MB‐231 cell migration and invasion, whereas carbon ion beam radiation suppressed these processes in a dose‐dependent manner. Depletion of SUN1 and SUN2 significantly suppressed X‐ray‐enhanced cell migration and invasion. Moreover, depletion or overexpression of each SUN1 splicing variant revealed that SUN1_888 containing 888 amino acids of SUN1 but not SUN1_916 was required for X‐ray‐enhanced migration and invasion. In addition, the results suggested that X‐ray irradiation affected the expression level of SUN1 splicing variants and a SUN protein binding partner, nesprins. Taken together, our findings supported that the LINC complex contributed to photon‐enhanced cell migration and invasion.
format Online
Article
Text
id pubmed-5891189
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-58911892018-04-13 X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex Imaizumi, Hiromasa Sato, Katsutoshi Nishihara, Asuka Minami, Kazumasa Koizumi, Masahiko Matsuura, Nariaki Hieda, Miki Cancer Sci Original Articles The linker of nucleoskeleton and cytoskeleton (LINC) complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, including nuclear migration, mechanotransduction, chromatin tethering and DNA damage response. We recently showed that a nuclear envelope protein, Sad1 and UNC84 domain protein 1 (SUN1), a component of the LINC complex, has a critical function in cell migration. Although ionizing radiation activates cell migration and invasion in vivo and in vitro, the underlying molecular mechanism remains unknown. Here, we examined the involvement of the LINC complex in radiation‐enhanced cell migration and invasion. A sublethal dose of X‐ray radiation promoted human breast cancer MDA‐MB‐231 cell migration and invasion, whereas carbon ion beam radiation suppressed these processes in a dose‐dependent manner. Depletion of SUN1 and SUN2 significantly suppressed X‐ray‐enhanced cell migration and invasion. Moreover, depletion or overexpression of each SUN1 splicing variant revealed that SUN1_888 containing 888 amino acids of SUN1 but not SUN1_916 was required for X‐ray‐enhanced migration and invasion. In addition, the results suggested that X‐ray irradiation affected the expression level of SUN1 splicing variants and a SUN protein binding partner, nesprins. Taken together, our findings supported that the LINC complex contributed to photon‐enhanced cell migration and invasion. John Wiley and Sons Inc. 2018-04-06 2018-04 /pmc/articles/PMC5891189/ /pubmed/29465769 http://dx.doi.org/10.1111/cas.13545 Text en © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Imaizumi, Hiromasa
Sato, Katsutoshi
Nishihara, Asuka
Minami, Kazumasa
Koizumi, Masahiko
Matsuura, Nariaki
Hieda, Miki
X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
title X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
title_full X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
title_fullStr X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
title_full_unstemmed X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
title_short X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
title_sort x‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891189/
https://www.ncbi.nlm.nih.gov/pubmed/29465769
http://dx.doi.org/10.1111/cas.13545
work_keys_str_mv AT imaizumihiromasa xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex
AT satokatsutoshi xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex
AT nishiharaasuka xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex
AT minamikazumasa xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex
AT koizumimasahiko xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex
AT matsuuranariaki xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex
AT hiedamiki xrayenhancedcancercellmigrationrequiresthelinkerofnucleoskeletonandcytoskeletoncomplex