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Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone
In the upper aerodigestive tract, biofilm deposits by oropharyngeal microbes can cause failure of medical polymer devices like voice prostheses. Previous studies on testing of inhibitive strategies still lack of comparability due to varying study protocols concerning growth media, microbial species...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891558/ https://www.ncbi.nlm.nih.gov/pubmed/29633010 http://dx.doi.org/10.1007/s10856-018-6051-7 |
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author | Leonhard, M. Zatorska, B. Moser, D. Tan, Y. Schneider-Stickler, B. |
author_facet | Leonhard, M. Zatorska, B. Moser, D. Tan, Y. Schneider-Stickler, B. |
author_sort | Leonhard, M. |
collection | PubMed |
description | In the upper aerodigestive tract, biofilm deposits by oropharyngeal microbes can cause failure of medical polymer devices like voice prostheses. Previous studies on testing of inhibitive strategies still lack of comparability due to varying study protocols concerning growth media, microbial species and growth conditions. Goal of the study was therefore to test cultivation of a mixed biofilm of isolated oropharyngeal microbes under in vitro growth conditions using mixtures of common growth media. Mixtures of yeast peptone dextrose medium (YPD), fetal bovine serum (FBS), RPMI 1640, Yeast nitrogen base medium (YNB) and brain heart infusion (BHI) were tested to grow mixed biofilm deposits of Candida albicans, Candida tropicalis, Staphylococcus aureus, Streptococcus epidermidis, Rothia dentocariosa and Lactobacillus gasseri on medical grade silicone. Periodic assessment of living biofilm was performed over 22 days by a digital microscope and the cultivated biofilm structures were analyzed by scanning electron microscopy after completion of the study. Mixtures of BHI, YPD and FBS improved microscopic growth of multispecies biofilm deposits over time, while addition of RPMI and YNB resulted in reduction of visible biofilm deposit sizes. A mixtures of FBS 30% + YPD 70% and BHI 30% + YPD 70% showed enhanced support of permanent surface growth on silicone. Growth kinetics of in vitro multispecies biofilms can be manipulated by using mixtures of common growth media. Using mixtures of growth media can improve growth of longterm multispecies oropharyngeal biofilm models used for in vitro testing of antibiofilm materials or coatings for voice prostheses. [Image: see text] |
format | Online Article Text |
id | pubmed-5891558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-58915582018-04-17 Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone Leonhard, M. Zatorska, B. Moser, D. Tan, Y. Schneider-Stickler, B. J Mater Sci Mater Med Biocompatibility Studies In the upper aerodigestive tract, biofilm deposits by oropharyngeal microbes can cause failure of medical polymer devices like voice prostheses. Previous studies on testing of inhibitive strategies still lack of comparability due to varying study protocols concerning growth media, microbial species and growth conditions. Goal of the study was therefore to test cultivation of a mixed biofilm of isolated oropharyngeal microbes under in vitro growth conditions using mixtures of common growth media. Mixtures of yeast peptone dextrose medium (YPD), fetal bovine serum (FBS), RPMI 1640, Yeast nitrogen base medium (YNB) and brain heart infusion (BHI) were tested to grow mixed biofilm deposits of Candida albicans, Candida tropicalis, Staphylococcus aureus, Streptococcus epidermidis, Rothia dentocariosa and Lactobacillus gasseri on medical grade silicone. Periodic assessment of living biofilm was performed over 22 days by a digital microscope and the cultivated biofilm structures were analyzed by scanning electron microscopy after completion of the study. Mixtures of BHI, YPD and FBS improved microscopic growth of multispecies biofilm deposits over time, while addition of RPMI and YNB resulted in reduction of visible biofilm deposit sizes. A mixtures of FBS 30% + YPD 70% and BHI 30% + YPD 70% showed enhanced support of permanent surface growth on silicone. Growth kinetics of in vitro multispecies biofilms can be manipulated by using mixtures of common growth media. Using mixtures of growth media can improve growth of longterm multispecies oropharyngeal biofilm models used for in vitro testing of antibiofilm materials or coatings for voice prostheses. [Image: see text] Springer US 2018-04-09 2018 /pmc/articles/PMC5891558/ /pubmed/29633010 http://dx.doi.org/10.1007/s10856-018-6051-7 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Biocompatibility Studies Leonhard, M. Zatorska, B. Moser, D. Tan, Y. Schneider-Stickler, B. Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
title | Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
title_full | Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
title_fullStr | Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
title_full_unstemmed | Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
title_short | Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
title_sort | evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone |
topic | Biocompatibility Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891558/ https://www.ncbi.nlm.nih.gov/pubmed/29633010 http://dx.doi.org/10.1007/s10856-018-6051-7 |
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