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The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus)
AIM: This research aimed to evaluate the effects of pomegranate extract standardized to 40% ellagic acid on the incised wound in albino rats. MATERIALS AND METHODS: Fifty albino rats were divided into 10 treatment groups. The five groups were sacrificed on the 8(th) day, while the others were sacrif...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Veterinary World
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891847/ https://www.ncbi.nlm.nih.gov/pubmed/29657424 http://dx.doi.org/10.14202/vetworld.2018.321-326 |
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author | Yuniarti, Wiwik Misaco Primarizky, Hardany Lukiswanto, Bambang Sektiari |
author_facet | Yuniarti, Wiwik Misaco Primarizky, Hardany Lukiswanto, Bambang Sektiari |
author_sort | Yuniarti, Wiwik Misaco |
collection | PubMed |
description | AIM: This research aimed to evaluate the effects of pomegranate extract standardized to 40% ellagic acid on the incised wound in albino rats. MATERIALS AND METHODS: Fifty albino rats were divided into 10 treatment groups. The five groups were sacrificed on the 8(th) day, while the others were sacrificed on the 15(th) day. Two groups of albino rats with incised wound were not treated at all (P0), the other two groups of albino rats with incised wound were treated with Betadine(®) (P1) ointment, and the rest of the groups were treated with pomegranate extract standardized to 40% ellagic acid with a concentration of 2.5% (P2), 5% (P3), and 7.5% (P4). The treatments were carried out twice a day with an interval of 12 h for 7 and 14 days. At the end of the research, the skin tissue of those albino rats had been taken for histopathologic preparations before H and E staining was performed. RESULTS: Collagen deposition, polymorphonuclear neutrophils (PMN) infiltration, angiogenesis, and fibrosis degree in Group P4 treated with 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days were significantly different from those in Groups P0, P1, P2, and P3, especially in the case of PMN inflammation (p<0.05). CONCLUSION: The administration of 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days on incised wounds of those albino rats can accelerate the wound healing process characterized by collagen deposition improvement, PMN infiltration in the wound area, angiogenesis, and fibrosis degree. |
format | Online Article Text |
id | pubmed-5891847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-58918472018-04-13 The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) Yuniarti, Wiwik Misaco Primarizky, Hardany Lukiswanto, Bambang Sektiari Vet World Research Article AIM: This research aimed to evaluate the effects of pomegranate extract standardized to 40% ellagic acid on the incised wound in albino rats. MATERIALS AND METHODS: Fifty albino rats were divided into 10 treatment groups. The five groups were sacrificed on the 8(th) day, while the others were sacrificed on the 15(th) day. Two groups of albino rats with incised wound were not treated at all (P0), the other two groups of albino rats with incised wound were treated with Betadine(®) (P1) ointment, and the rest of the groups were treated with pomegranate extract standardized to 40% ellagic acid with a concentration of 2.5% (P2), 5% (P3), and 7.5% (P4). The treatments were carried out twice a day with an interval of 12 h for 7 and 14 days. At the end of the research, the skin tissue of those albino rats had been taken for histopathologic preparations before H and E staining was performed. RESULTS: Collagen deposition, polymorphonuclear neutrophils (PMN) infiltration, angiogenesis, and fibrosis degree in Group P4 treated with 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days were significantly different from those in Groups P0, P1, P2, and P3, especially in the case of PMN inflammation (p<0.05). CONCLUSION: The administration of 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days on incised wounds of those albino rats can accelerate the wound healing process characterized by collagen deposition improvement, PMN infiltration in the wound area, angiogenesis, and fibrosis degree. Veterinary World 2018-03 2018-03-17 /pmc/articles/PMC5891847/ /pubmed/29657424 http://dx.doi.org/10.14202/vetworld.2018.321-326 Text en Copyright: © Yuniarti, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yuniarti, Wiwik Misaco Primarizky, Hardany Lukiswanto, Bambang Sektiari The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) |
title | The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) |
title_full | The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) |
title_fullStr | The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) |
title_full_unstemmed | The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) |
title_short | The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus) |
title_sort | activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (rattus norvegicus) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891847/ https://www.ncbi.nlm.nih.gov/pubmed/29657424 http://dx.doi.org/10.14202/vetworld.2018.321-326 |
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