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Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria
AIM: This work aimed to evaluate the biological activity of the leaf extracts of Juniperus thurifera L., which is an Algerian endemic tree that belongs to the family of Cupressaceae. MATERIALS AND METHODS: The plant leaves were extracted in solvents of increasing polarity to obtain different extract...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Veterinary World
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891855/ https://www.ncbi.nlm.nih.gov/pubmed/29657432 http://dx.doi.org/10.14202/vetworld.2018.373-378 |
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author | Manel, Merradi Nouzha, Heleili Rim, Mekari Imane, Mekkaoui Sana, Aouachria Yasmine, Oucheriah Ammar, Ayachi |
author_facet | Manel, Merradi Nouzha, Heleili Rim, Mekari Imane, Mekkaoui Sana, Aouachria Yasmine, Oucheriah Ammar, Ayachi |
author_sort | Manel, Merradi |
collection | PubMed |
description | AIM: This work aimed to evaluate the biological activity of the leaf extracts of Juniperus thurifera L., which is an Algerian endemic tree that belongs to the family of Cupressaceae. MATERIALS AND METHODS: The plant leaves were extracted in solvents of increasing polarity to obtain different extracts such as methanol, petroleum ether, chloroform, ethyl acetate, and aqueous extracts (MeE, PEE, ChlE, EtAE, and AqE). The antioxidant activity of four extracts (MeE, ChlE, EtAE, and AqE) was assessed by trapping test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The evaluation of antibacterial activity of MeE, ChlE, EtAE, and PEE was done using the disk diffusion method on solid agar. RESULTS: The three extracts of EtAE, AqE, and MeE showed high antiradical activity toward the DPPH radical (IC(50)=29.348 µg/mL, 37.538 µg/mL, and 52.573 µg/mL, respectively), while the lowest radical scavenging activity was expressed by the ChlE (IC(50)=70.096 µg/mL). These extracts were active only toward the Gram-positive bacteria (Staphylococcus aureus ATCC and methicillin-resistant S. aureus) at different concentrations, and the highest activity was obtained with the ChlE with an inhibition diameter of 14 mm at the concentration of 1 g/mL. No inhibition was detected for all of these extracts against the Gram-negative tested strains (Escherichia coli ATCC, Pseudomonas aeruginosa ATCC, and Enterobacter cloacae (extended spectrum β-lactamase). CONCLUSION: From this study, on the one hand, it was concluded that J. thurifera L. leaves extracts exhibited a very intense antioxidant potential toward the DPPH radical, and on the other hand, the antibacterial activity showed an action spectrum exclusively toward the Gram-positive bacteria. |
format | Online Article Text |
id | pubmed-5891855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-58918552018-04-13 Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria Manel, Merradi Nouzha, Heleili Rim, Mekari Imane, Mekkaoui Sana, Aouachria Yasmine, Oucheriah Ammar, Ayachi Vet World Research Article AIM: This work aimed to evaluate the biological activity of the leaf extracts of Juniperus thurifera L., which is an Algerian endemic tree that belongs to the family of Cupressaceae. MATERIALS AND METHODS: The plant leaves were extracted in solvents of increasing polarity to obtain different extracts such as methanol, petroleum ether, chloroform, ethyl acetate, and aqueous extracts (MeE, PEE, ChlE, EtAE, and AqE). The antioxidant activity of four extracts (MeE, ChlE, EtAE, and AqE) was assessed by trapping test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The evaluation of antibacterial activity of MeE, ChlE, EtAE, and PEE was done using the disk diffusion method on solid agar. RESULTS: The three extracts of EtAE, AqE, and MeE showed high antiradical activity toward the DPPH radical (IC(50)=29.348 µg/mL, 37.538 µg/mL, and 52.573 µg/mL, respectively), while the lowest radical scavenging activity was expressed by the ChlE (IC(50)=70.096 µg/mL). These extracts were active only toward the Gram-positive bacteria (Staphylococcus aureus ATCC and methicillin-resistant S. aureus) at different concentrations, and the highest activity was obtained with the ChlE with an inhibition diameter of 14 mm at the concentration of 1 g/mL. No inhibition was detected for all of these extracts against the Gram-negative tested strains (Escherichia coli ATCC, Pseudomonas aeruginosa ATCC, and Enterobacter cloacae (extended spectrum β-lactamase). CONCLUSION: From this study, on the one hand, it was concluded that J. thurifera L. leaves extracts exhibited a very intense antioxidant potential toward the DPPH radical, and on the other hand, the antibacterial activity showed an action spectrum exclusively toward the Gram-positive bacteria. Veterinary World 2018-03 2018-03-27 /pmc/articles/PMC5891855/ /pubmed/29657432 http://dx.doi.org/10.14202/vetworld.2018.373-378 Text en Copyright: © Manel, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Manel, Merradi Nouzha, Heleili Rim, Mekari Imane, Mekkaoui Sana, Aouachria Yasmine, Oucheriah Ammar, Ayachi Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria |
title | Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria |
title_full | Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria |
title_fullStr | Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria |
title_full_unstemmed | Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria |
title_short | Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria |
title_sort | antibacterial and antioxidant activity of juniperus thurifera l. leaf extracts growing in east of algeria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891855/ https://www.ncbi.nlm.nih.gov/pubmed/29657432 http://dx.doi.org/10.14202/vetworld.2018.373-378 |
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