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Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer

Recently, artificial life has been created with artificial materials and methods. Life can be created when genomic DNA molecules are integrated in liposomes containing biochemical reactions for biogenic needs. However, it is not yet known whether the integration of these parts will be able to occur...

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Autor principal: Kobayashi, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892112/
https://www.ncbi.nlm.nih.gov/pubmed/29463604
http://dx.doi.org/10.1128/JB.00630-17
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author Kobayashi, Hideki
author_facet Kobayashi, Hideki
author_sort Kobayashi, Hideki
collection PubMed
description Recently, artificial life has been created with artificial materials and methods. Life can be created when genomic DNA molecules are integrated in liposomes containing biochemical reactions for biogenic needs. However, it is not yet known whether the integration of these parts will be able to occur in nature and constitute a living system. I planned to regenerate bacteria from biologically active liposomes by inserting genomic DNA using only natural materials and methods. Minicells of Escherichia coli, containing plasmids and activated SOS proteins, act as protocells. Four new E. coli strains were regenerated from minicells by inserting the genomes by using the system for conjugation between F(−) and Hfr strains. Cells of the four regenerated strains showed the same genetic markers as the two genome donors. Pulse-field gel electrophoresis of their genomes showed admixing of those of both donors. In addition, the genomes of the four regenerated strains had chimeric genome of the two donors. These results show that synthesis of life can occur in nature without artificial arrangement. IMPORTANCE What is the difference between inanimate objects and organisms? Organisms always have genomic DNA. When organisms lose their genomes, they can neither grow nor reproduce. As the result, organisms turn into inanimate objects without their genomes. In this study, I regenerated microbes from cells that had lost their genomes (cell corpses) by inserting another genome. All steps of regeneration used the natural behavior of microbes. The same regeneration of microbes could happen in nature. These primitive lives have plasticity, which accelerates evolution and provides various kinds of life in the world.
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spelling pubmed-58921122018-04-13 Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer Kobayashi, Hideki J Bacteriol Research Article Recently, artificial life has been created with artificial materials and methods. Life can be created when genomic DNA molecules are integrated in liposomes containing biochemical reactions for biogenic needs. However, it is not yet known whether the integration of these parts will be able to occur in nature and constitute a living system. I planned to regenerate bacteria from biologically active liposomes by inserting genomic DNA using only natural materials and methods. Minicells of Escherichia coli, containing plasmids and activated SOS proteins, act as protocells. Four new E. coli strains were regenerated from minicells by inserting the genomes by using the system for conjugation between F(−) and Hfr strains. Cells of the four regenerated strains showed the same genetic markers as the two genome donors. Pulse-field gel electrophoresis of their genomes showed admixing of those of both donors. In addition, the genomes of the four regenerated strains had chimeric genome of the two donors. These results show that synthesis of life can occur in nature without artificial arrangement. IMPORTANCE What is the difference between inanimate objects and organisms? Organisms always have genomic DNA. When organisms lose their genomes, they can neither grow nor reproduce. As the result, organisms turn into inanimate objects without their genomes. In this study, I regenerated microbes from cells that had lost their genomes (cell corpses) by inserting another genome. All steps of regeneration used the natural behavior of microbes. The same regeneration of microbes could happen in nature. These primitive lives have plasticity, which accelerates evolution and provides various kinds of life in the world. American Society for Microbiology 2018-04-09 /pmc/articles/PMC5892112/ /pubmed/29463604 http://dx.doi.org/10.1128/JB.00630-17 Text en Copyright © 2018 Kobayashi. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Kobayashi, Hideki
Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
title Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
title_full Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
title_fullStr Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
title_full_unstemmed Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
title_short Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
title_sort regeneration of escherichia coli from minicells through lateral gene transfer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892112/
https://www.ncbi.nlm.nih.gov/pubmed/29463604
http://dx.doi.org/10.1128/JB.00630-17
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