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Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy

The rational design of robust fluorescent organic materials for long-term cell tracing is still challenging, and aggregation-caused quenching of emission is a big limitation of this strategy. Organic dyes with aggregation-induced emission (AIE) can effectively address this problem. Herein, AIEgen-co...

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Detalles Bibliográficos
Autores principales: Zhang, Jianxu, Xu, Bin, Tian, Wenjing, Xie, Zhigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892346/
https://www.ncbi.nlm.nih.gov/pubmed/29675254
http://dx.doi.org/10.1039/c7sc05130a
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author Zhang, Jianxu
Xu, Bin
Tian, Wenjing
Xie, Zhigang
author_facet Zhang, Jianxu
Xu, Bin
Tian, Wenjing
Xie, Zhigang
author_sort Zhang, Jianxu
collection PubMed
description The rational design of robust fluorescent organic materials for long-term cell tracing is still challenging, and aggregation-caused quenching of emission is a big limitation of this strategy. Organic dyes with aggregation-induced emission (AIE) can effectively address this problem. Herein, AIEgen-containing nanoparticles, with different morphologies and emission, were prepared by assembling amphiphilic copolymers with an AIEgen. We compared the physical and chemical properties of rod-like and spherical nanoparticles, particularly investigating the effects of the shape on internalization and the imaging effect. The formulated nanoparticles exhibit advantageous features, such as a large Stokes shift, robust stability in physiological conditions, strong fluorescent emission, and photobleaching resistance. Interestingly, the rod-like nanoparticles were internalized more efficiently than their spherical counterparts, and their strong green fluorescence can still be clearly observed even after 15 days in vitro and in vivo. This work demonstrates the great potential of regulating the morphology of nanoparticles to obtain an ideal biological function.
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spelling pubmed-58923462018-04-19 Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy Zhang, Jianxu Xu, Bin Tian, Wenjing Xie, Zhigang Chem Sci Chemistry The rational design of robust fluorescent organic materials for long-term cell tracing is still challenging, and aggregation-caused quenching of emission is a big limitation of this strategy. Organic dyes with aggregation-induced emission (AIE) can effectively address this problem. Herein, AIEgen-containing nanoparticles, with different morphologies and emission, were prepared by assembling amphiphilic copolymers with an AIEgen. We compared the physical and chemical properties of rod-like and spherical nanoparticles, particularly investigating the effects of the shape on internalization and the imaging effect. The formulated nanoparticles exhibit advantageous features, such as a large Stokes shift, robust stability in physiological conditions, strong fluorescent emission, and photobleaching resistance. Interestingly, the rod-like nanoparticles were internalized more efficiently than their spherical counterparts, and their strong green fluorescence can still be clearly observed even after 15 days in vitro and in vivo. This work demonstrates the great potential of regulating the morphology of nanoparticles to obtain an ideal biological function. Royal Society of Chemistry 2018-01-17 /pmc/articles/PMC5892346/ /pubmed/29675254 http://dx.doi.org/10.1039/c7sc05130a Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Zhang, Jianxu
Xu, Bin
Tian, Wenjing
Xie, Zhigang
Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
title Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
title_full Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
title_fullStr Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
title_full_unstemmed Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
title_short Tailoring the morphology of AIEgen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
title_sort tailoring the morphology of aiegen fluorescent nanoparticles for optimal cellular uptake and imaging efficacy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892346/
https://www.ncbi.nlm.nih.gov/pubmed/29675254
http://dx.doi.org/10.1039/c7sc05130a
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