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A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells

The Golgi apparatus is well-known as the center of vesicle trafficking whose malfunction might cause the breakdown of overall cellular architecture and ultimately cell death. The development of fluorescent probes to not only precisely stain the Golgi apparatus but also monitor dynamic vesicle traffi...

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Detalles Bibliográficos
Autores principales: Tang, Juan, Yin, Hao-Yan, Zhang, Jun-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892405/
https://www.ncbi.nlm.nih.gov/pubmed/29675239
http://dx.doi.org/10.1039/c7sc04498d
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author Tang, Juan
Yin, Hao-Yan
Zhang, Jun-Long
author_facet Tang, Juan
Yin, Hao-Yan
Zhang, Jun-Long
author_sort Tang, Juan
collection PubMed
description The Golgi apparatus is well-known as the center of vesicle trafficking whose malfunction might cause the breakdown of overall cellular architecture and ultimately cell death. The development of fluorescent probes to not only precisely stain the Golgi apparatus but also monitor dynamic vesicle trafficking is of great significance. While fluorescent proteins and fluorescent lipid analogs have been reported, they are sometime limited by either overexpression and toxicity or lack of high selectivity, respectively. We herein report a novel approach based on metal-induced coordination between the phosphate anions of phospholipids and the metal center of a luminescent Alsalen complex AlL, which can in situ track membrane vesicle trafficking from the Golgi apparatus to the lysosomes in living cells. This work opens a new avenue for designing luminescent metal probes based on the Lewis acidity of metal ions and allows the use of metal ions with different charge states, polarities, and reactivities within a similar structural scaffold to expand coordination chemistry for biological studies.
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spelling pubmed-58924052018-04-19 A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells Tang, Juan Yin, Hao-Yan Zhang, Jun-Long Chem Sci Chemistry The Golgi apparatus is well-known as the center of vesicle trafficking whose malfunction might cause the breakdown of overall cellular architecture and ultimately cell death. The development of fluorescent probes to not only precisely stain the Golgi apparatus but also monitor dynamic vesicle trafficking is of great significance. While fluorescent proteins and fluorescent lipid analogs have been reported, they are sometime limited by either overexpression and toxicity or lack of high selectivity, respectively. We herein report a novel approach based on metal-induced coordination between the phosphate anions of phospholipids and the metal center of a luminescent Alsalen complex AlL, which can in situ track membrane vesicle trafficking from the Golgi apparatus to the lysosomes in living cells. This work opens a new avenue for designing luminescent metal probes based on the Lewis acidity of metal ions and allows the use of metal ions with different charge states, polarities, and reactivities within a similar structural scaffold to expand coordination chemistry for biological studies. Royal Society of Chemistry 2018-01-08 /pmc/articles/PMC5892405/ /pubmed/29675239 http://dx.doi.org/10.1039/c7sc04498d Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Tang, Juan
Yin, Hao-Yan
Zhang, Jun-Long
A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells
title A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells
title_full A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells
title_fullStr A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells
title_full_unstemmed A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells
title_short A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells
title_sort luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the golgi apparatus to lysosomes in living cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892405/
https://www.ncbi.nlm.nih.gov/pubmed/29675239
http://dx.doi.org/10.1039/c7sc04498d
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