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Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum
BACKGROUND: Although elemental selenium has been found to be effective against Eimeria, no study has yet investigated the effects of selenium nanoparticles (SeNPs) on the Eimeria parasite. The aim of this study, therefore, was to evaluate the ameliorative effect of SeNPs compared with elemental sele...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892949/ https://www.ncbi.nlm.nih.gov/pubmed/29662312 http://dx.doi.org/10.2147/IJN.S162355 |
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author | Alkhudhayri, Abdulsalam A Dkhil, Mohamed A Al-Quraishy, Saleh |
author_facet | Alkhudhayri, Abdulsalam A Dkhil, Mohamed A Al-Quraishy, Saleh |
author_sort | Alkhudhayri, Abdulsalam A |
collection | PubMed |
description | BACKGROUND: Although elemental selenium has been found to be effective against Eimeria, no study has yet investigated the effects of selenium nanoparticles (SeNPs) on the Eimeria parasite. The aim of this study, therefore, was to evaluate the ameliorative effect of SeNPs compared with elemental selenium on mice jejunum infected with sporulated oocysts of Eimeria papillata. METHODS: The mice were divided into 4 groups, with the first being the non-infected, control group, and the second, third, and fourth groups being orally inoculated with 1,000 sporulated oocysts of E. papillata. The third and fourth groups also received, respectively, an oral dose of 0.1 mg/kg sodium selenite and 0.5 mg/kg SeNPs daily for 5 consecutive days. RESULTS: The infection induced severe histopathological jejunal damage, reflected in the form of destroyed jejunal mucosa, increased jejunal oxidative damage, a reduction in the number of jejunal goblet cells, and increased production of pro-inflammatory cytokines, quantified by real-time polymerase chain reaction. Treatment of mice with SeNPs significantly decreased the oocyst output in the feces by ~80%. Furthermore, the number of parasitic stages counted in stained jejunal paraffin sections was significantly decreased after the mice were treated with SeNPs. In addition, the number of goblet cells increased from 42.6±7.3 to 95.3±8.5 cells/10 villus-crypt units after treatment. By day 5 post-infection with E. papillata, SeNPs could be seen to have significantly increased the activity of glutathione peroxidase from 263±10 to 402.4±9 mU/mL. Finally, SeNPs were able to regulate the gene expression of mucin 2, interleukin 1β, interleukin 6, interferon-γ, and tumor necrosis factor α in the jejunum of mice infected with E. papillata. CONCLUSION: The results collectively showed that SeNPs are more effective than sodium selenite with regard to their anti-coccidial, anti-oxidant, and anti-inflammatory role against eimeriosis induced in the jejunum of mice. |
format | Online Article Text |
id | pubmed-5892949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58929492018-04-16 Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum Alkhudhayri, Abdulsalam A Dkhil, Mohamed A Al-Quraishy, Saleh Int J Nanomedicine Original Research BACKGROUND: Although elemental selenium has been found to be effective against Eimeria, no study has yet investigated the effects of selenium nanoparticles (SeNPs) on the Eimeria parasite. The aim of this study, therefore, was to evaluate the ameliorative effect of SeNPs compared with elemental selenium on mice jejunum infected with sporulated oocysts of Eimeria papillata. METHODS: The mice were divided into 4 groups, with the first being the non-infected, control group, and the second, third, and fourth groups being orally inoculated with 1,000 sporulated oocysts of E. papillata. The third and fourth groups also received, respectively, an oral dose of 0.1 mg/kg sodium selenite and 0.5 mg/kg SeNPs daily for 5 consecutive days. RESULTS: The infection induced severe histopathological jejunal damage, reflected in the form of destroyed jejunal mucosa, increased jejunal oxidative damage, a reduction in the number of jejunal goblet cells, and increased production of pro-inflammatory cytokines, quantified by real-time polymerase chain reaction. Treatment of mice with SeNPs significantly decreased the oocyst output in the feces by ~80%. Furthermore, the number of parasitic stages counted in stained jejunal paraffin sections was significantly decreased after the mice were treated with SeNPs. In addition, the number of goblet cells increased from 42.6±7.3 to 95.3±8.5 cells/10 villus-crypt units after treatment. By day 5 post-infection with E. papillata, SeNPs could be seen to have significantly increased the activity of glutathione peroxidase from 263±10 to 402.4±9 mU/mL. Finally, SeNPs were able to regulate the gene expression of mucin 2, interleukin 1β, interleukin 6, interferon-γ, and tumor necrosis factor α in the jejunum of mice infected with E. papillata. CONCLUSION: The results collectively showed that SeNPs are more effective than sodium selenite with regard to their anti-coccidial, anti-oxidant, and anti-inflammatory role against eimeriosis induced in the jejunum of mice. Dove Medical Press 2018-04-03 /pmc/articles/PMC5892949/ /pubmed/29662312 http://dx.doi.org/10.2147/IJN.S162355 Text en © 2018 Alkhudhayri et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Alkhudhayri, Abdulsalam A Dkhil, Mohamed A Al-Quraishy, Saleh Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
title | Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
title_full | Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
title_fullStr | Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
title_full_unstemmed | Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
title_short | Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
title_sort | nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892949/ https://www.ncbi.nlm.nih.gov/pubmed/29662312 http://dx.doi.org/10.2147/IJN.S162355 |
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