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Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection

Human papillomavirus (HPV) infection is a frequent cause of malignant and non-malignant disease, in particular among persons with HIV. HPV serotype-specific anti L1 antibodies protect against HPV infection but little is known about prophylactic HPV vaccine-induced cell-mediated immunity against HPV...

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Autores principales: Zurek Munk-Madsen, Maria, Toft, Lars, Kube, Tina, Richter, Rolf, Ostergaard, Lars, Søgaard, Ole S., Tolstrup, Martin, Kaufmann, Andreas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893199/
https://www.ncbi.nlm.nih.gov/pubmed/29172992
http://dx.doi.org/10.1080/21645515.2017.1407896
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author Zurek Munk-Madsen, Maria
Toft, Lars
Kube, Tina
Richter, Rolf
Ostergaard, Lars
Søgaard, Ole S.
Tolstrup, Martin
Kaufmann, Andreas M.
author_facet Zurek Munk-Madsen, Maria
Toft, Lars
Kube, Tina
Richter, Rolf
Ostergaard, Lars
Søgaard, Ole S.
Tolstrup, Martin
Kaufmann, Andreas M.
author_sort Zurek Munk-Madsen, Maria
collection PubMed
description Human papillomavirus (HPV) infection is a frequent cause of malignant and non-malignant disease, in particular among persons with HIV. HPV serotype-specific anti L1 antibodies protect against HPV infection but little is known about prophylactic HPV vaccine-induced cell-mediated immunity against HPV in high-risk individuals. We recently showed that both HPV vaccines (Gardasil® and Cervarix®) induce solid, serological immune responses in HIV-infected persons. This study aimed to characterize HPV-specific CD4 T cells in HIV-infected HPV-vaccine recipients, T cell responses being critical for B cell activation and antibody-isotype switching. Thirty HIV-infected patients on long-term antiretroviral treatment (ART) received 3 doses of either Cervarix (n = 15) or Gardasil (n = 15) vaccine at month 0, 1.5 and 6. Cryopreserved peripheral blood mononuclear cells (PBMC) from baseline, 7 and 12 months were subjected to 24-hour stimulation with specific pools of HPV L1-peptides (HPV6, 11, 16, 18, 31 and 45) and HPV E6/E7-peptide pools (HPV6/11 and HPV16/18). Fluorescence-activated cell sorting with intracellular staining (IC-FACS) against CD4, CD154, IL-2, and IFNγ was performed. Frequencies (%) of HPV-antigen specific CD4+ T cells (CD154(+)/IL-2(+) or CD154(+)/ IFNγ(+)) were determined. Both HPV-vaccines significantly and comparably enhanced cell-mediated vaccine L1 antigen-specific immunity in HIV-positive adults receiving ART therapy at month 7 and 12 after first vaccine dose. This suggests that the vaccines induce CD4 T cellular memory despite HIV-induced immune compromisation.
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spelling pubmed-58931992018-04-13 Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection Zurek Munk-Madsen, Maria Toft, Lars Kube, Tina Richter, Rolf Ostergaard, Lars Søgaard, Ole S. Tolstrup, Martin Kaufmann, Andreas M. Hum Vaccin Immunother Research Paper Human papillomavirus (HPV) infection is a frequent cause of malignant and non-malignant disease, in particular among persons with HIV. HPV serotype-specific anti L1 antibodies protect against HPV infection but little is known about prophylactic HPV vaccine-induced cell-mediated immunity against HPV in high-risk individuals. We recently showed that both HPV vaccines (Gardasil® and Cervarix®) induce solid, serological immune responses in HIV-infected persons. This study aimed to characterize HPV-specific CD4 T cells in HIV-infected HPV-vaccine recipients, T cell responses being critical for B cell activation and antibody-isotype switching. Thirty HIV-infected patients on long-term antiretroviral treatment (ART) received 3 doses of either Cervarix (n = 15) or Gardasil (n = 15) vaccine at month 0, 1.5 and 6. Cryopreserved peripheral blood mononuclear cells (PBMC) from baseline, 7 and 12 months were subjected to 24-hour stimulation with specific pools of HPV L1-peptides (HPV6, 11, 16, 18, 31 and 45) and HPV E6/E7-peptide pools (HPV6/11 and HPV16/18). Fluorescence-activated cell sorting with intracellular staining (IC-FACS) against CD4, CD154, IL-2, and IFNγ was performed. Frequencies (%) of HPV-antigen specific CD4+ T cells (CD154(+)/IL-2(+) or CD154(+)/ IFNγ(+)) were determined. Both HPV-vaccines significantly and comparably enhanced cell-mediated vaccine L1 antigen-specific immunity in HIV-positive adults receiving ART therapy at month 7 and 12 after first vaccine dose. This suggests that the vaccines induce CD4 T cellular memory despite HIV-induced immune compromisation. Taylor & Francis 2017-12-20 /pmc/articles/PMC5893199/ /pubmed/29172992 http://dx.doi.org/10.1080/21645515.2017.1407896 Text en © 2018 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Research Paper
Zurek Munk-Madsen, Maria
Toft, Lars
Kube, Tina
Richter, Rolf
Ostergaard, Lars
Søgaard, Ole S.
Tolstrup, Martin
Kaufmann, Andreas M.
Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection
title Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection
title_full Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection
title_fullStr Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection
title_full_unstemmed Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection
title_short Cellular immunogenicity of human papillomavirus vaccines Cervarix and Gardasil in adults with HIV infection
title_sort cellular immunogenicity of human papillomavirus vaccines cervarix and gardasil in adults with hiv infection
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893199/
https://www.ncbi.nlm.nih.gov/pubmed/29172992
http://dx.doi.org/10.1080/21645515.2017.1407896
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