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MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells

OBJECTIVE: In order to clarify the role of microRNAs (miRNA) in megakaryocyte differentiation, we ran a microRNA microarray experiment to measure the expression level of 961 human miRNA in megakaryocytes differentiated from human umbilical cord blood CD133+ cells. MATERIALS AND METHODS: In this expe...

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Autores principales: Houshmand, Mohammad, Nakhlestani Hagh, Mozhde, Soleimani, Masoud, Hamidieh, Amir Ali, Abroun, Saeed, Nikougoftar Zarif, Mahin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893291/
https://www.ncbi.nlm.nih.gov/pubmed/29633597
http://dx.doi.org/10.22074/cellj.2018.5021
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author Houshmand, Mohammad
Nakhlestani Hagh, Mozhde
Soleimani, Masoud
Hamidieh, Amir Ali
Abroun, Saeed
Nikougoftar Zarif, Mahin
author_facet Houshmand, Mohammad
Nakhlestani Hagh, Mozhde
Soleimani, Masoud
Hamidieh, Amir Ali
Abroun, Saeed
Nikougoftar Zarif, Mahin
author_sort Houshmand, Mohammad
collection PubMed
description OBJECTIVE: In order to clarify the role of microRNAs (miRNA) in megakaryocyte differentiation, we ran a microRNA microarray experiment to measure the expression level of 961 human miRNA in megakaryocytes differentiated from human umbilical cord blood CD133+ cells. MATERIALS AND METHODS: In this experimental study, human CD133+ hematopoietic stem cells were collected from three human umbilical cord blood (UCB) samples, and then differentiated to the megakaryocytic lineage and characterized by flow cytometry, CFU-assay and ploidy analysis. Subsequently, microarray analysis was undertaken followed by quantitative polymerase chain reaction (qPCR) to validate differentially expressed miRNA identified in the microarray analysis. RESULTS: A total of 10 and 14 miRNAs were upregulated (e.g. miR-1246 and miR-148-a) and down-regulated (e.g. miR- 551b and miR-10a) respectively during megakaryocyte differentiation, all of which were confirmed by qPCR. Analysis of targets of these miRNA showed that the majority of targets are transcription factors involved in megakaryopoiesis. CONCLUSION: We conclude that miRNA play an important role in megakaryocyte differentiation and may be used as targets to change the rate of differentiation and further our understanding of the biology of megakaryocyte commitment.
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spelling pubmed-58932912018-07-01 MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells Houshmand, Mohammad Nakhlestani Hagh, Mozhde Soleimani, Masoud Hamidieh, Amir Ali Abroun, Saeed Nikougoftar Zarif, Mahin Cell J Original Article OBJECTIVE: In order to clarify the role of microRNAs (miRNA) in megakaryocyte differentiation, we ran a microRNA microarray experiment to measure the expression level of 961 human miRNA in megakaryocytes differentiated from human umbilical cord blood CD133+ cells. MATERIALS AND METHODS: In this experimental study, human CD133+ hematopoietic stem cells were collected from three human umbilical cord blood (UCB) samples, and then differentiated to the megakaryocytic lineage and characterized by flow cytometry, CFU-assay and ploidy analysis. Subsequently, microarray analysis was undertaken followed by quantitative polymerase chain reaction (qPCR) to validate differentially expressed miRNA identified in the microarray analysis. RESULTS: A total of 10 and 14 miRNAs were upregulated (e.g. miR-1246 and miR-148-a) and down-regulated (e.g. miR- 551b and miR-10a) respectively during megakaryocyte differentiation, all of which were confirmed by qPCR. Analysis of targets of these miRNA showed that the majority of targets are transcription factors involved in megakaryopoiesis. CONCLUSION: We conclude that miRNA play an important role in megakaryocyte differentiation and may be used as targets to change the rate of differentiation and further our understanding of the biology of megakaryocyte commitment. Royan Institute 2018 2018-03-18 /pmc/articles/PMC5893291/ /pubmed/29633597 http://dx.doi.org/10.22074/cellj.2018.5021 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Houshmand, Mohammad
Nakhlestani Hagh, Mozhde
Soleimani, Masoud
Hamidieh, Amir Ali
Abroun, Saeed
Nikougoftar Zarif, Mahin
MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells
title MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells
title_full MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells
title_fullStr MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells
title_full_unstemmed MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells
title_short MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells
title_sort microrna microarray profiling during megakaryocyte differentiation of cord blood cd133+ hematopoietic stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893291/
https://www.ncbi.nlm.nih.gov/pubmed/29633597
http://dx.doi.org/10.22074/cellj.2018.5021
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