A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis

Nitrate is a nutrient signal that triggers complex regulation of transcriptional networks to modulate nutrient-dependent growth and development in plants. This includes time- and nitrate concentration-dependent regulation of nitrate-related gene expression. However, the underlying mechanisms remain...

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Autores principales: Maeda, Yoshie, Konishi, Mineko, Kiba, Takatoshi, Sakuraba, Yasuhito, Sawaki, Naoya, Kurai, Tomohiro, Ueda, Yoshiaki, Sakakibara, Hitoshi, Yanagisawa, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893545/
https://www.ncbi.nlm.nih.gov/pubmed/29636481
http://dx.doi.org/10.1038/s41467-018-03832-6
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author Maeda, Yoshie
Konishi, Mineko
Kiba, Takatoshi
Sakuraba, Yasuhito
Sawaki, Naoya
Kurai, Tomohiro
Ueda, Yoshiaki
Sakakibara, Hitoshi
Yanagisawa, Shuichi
author_facet Maeda, Yoshie
Konishi, Mineko
Kiba, Takatoshi
Sakuraba, Yasuhito
Sawaki, Naoya
Kurai, Tomohiro
Ueda, Yoshiaki
Sakakibara, Hitoshi
Yanagisawa, Shuichi
author_sort Maeda, Yoshie
collection PubMed
description Nitrate is a nutrient signal that triggers complex regulation of transcriptional networks to modulate nutrient-dependent growth and development in plants. This includes time- and nitrate concentration-dependent regulation of nitrate-related gene expression. However, the underlying mechanisms remain poorly understood. Here we identify NIGT1 transcriptional repressors as negative regulators of the Arabidopsis NRT2.1 nitrate transporter gene, and show antagonistic regulation by NLP primary transcription factors for nitrate signalling and the NLP-NIGT1 transcriptional cascade-mediated repression. This antagonistic regulation provides a resolution to the complexity of nitrate-induced transcriptional regulations. Genome-wide analysis reveals that this mechanism is applicable to NRT2.1 and other genes involved in nitrate assimilation, hormone biosynthesis and transcription. Furthermore, the PHR1 master regulator of the phosphorus-starvation response also directly promotes expression of NIGT1 family genes, leading to reductions in nitrate uptake. NIGT1 repressors thus act in two transcriptional cascades, forming a direct link between phosphorus and nitrogen nutritional regulation.
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spelling pubmed-58935452018-04-13 A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis Maeda, Yoshie Konishi, Mineko Kiba, Takatoshi Sakuraba, Yasuhito Sawaki, Naoya Kurai, Tomohiro Ueda, Yoshiaki Sakakibara, Hitoshi Yanagisawa, Shuichi Nat Commun Article Nitrate is a nutrient signal that triggers complex regulation of transcriptional networks to modulate nutrient-dependent growth and development in plants. This includes time- and nitrate concentration-dependent regulation of nitrate-related gene expression. However, the underlying mechanisms remain poorly understood. Here we identify NIGT1 transcriptional repressors as negative regulators of the Arabidopsis NRT2.1 nitrate transporter gene, and show antagonistic regulation by NLP primary transcription factors for nitrate signalling and the NLP-NIGT1 transcriptional cascade-mediated repression. This antagonistic regulation provides a resolution to the complexity of nitrate-induced transcriptional regulations. Genome-wide analysis reveals that this mechanism is applicable to NRT2.1 and other genes involved in nitrate assimilation, hormone biosynthesis and transcription. Furthermore, the PHR1 master regulator of the phosphorus-starvation response also directly promotes expression of NIGT1 family genes, leading to reductions in nitrate uptake. NIGT1 repressors thus act in two transcriptional cascades, forming a direct link between phosphorus and nitrogen nutritional regulation. Nature Publishing Group UK 2018-04-10 /pmc/articles/PMC5893545/ /pubmed/29636481 http://dx.doi.org/10.1038/s41467-018-03832-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Maeda, Yoshie
Konishi, Mineko
Kiba, Takatoshi
Sakuraba, Yasuhito
Sawaki, Naoya
Kurai, Tomohiro
Ueda, Yoshiaki
Sakakibara, Hitoshi
Yanagisawa, Shuichi
A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis
title A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis
title_full A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis
title_fullStr A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis
title_full_unstemmed A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis
title_short A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis
title_sort nigt1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893545/
https://www.ncbi.nlm.nih.gov/pubmed/29636481
http://dx.doi.org/10.1038/s41467-018-03832-6
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