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A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production
Bacterial cellulose (BC) is a biocompatible material with versatile applications. However, its large-scale production is challenged by the limited biological knowledge of the bacteria. The advent of synthetic biology has lead the way to the development of BC producing microbes as a novel chassis. He...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893554/ https://www.ncbi.nlm.nih.gov/pubmed/29636541 http://dx.doi.org/10.1038/s41598-018-23701-y |
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author | Basu, Anindya Vadanan, Sundaravadanam Vishnu Lim, Sierin |
author_facet | Basu, Anindya Vadanan, Sundaravadanam Vishnu Lim, Sierin |
author_sort | Basu, Anindya |
collection | PubMed |
description | Bacterial cellulose (BC) is a biocompatible material with versatile applications. However, its large-scale production is challenged by the limited biological knowledge of the bacteria. The advent of synthetic biology has lead the way to the development of BC producing microbes as a novel chassis. Hence, investigation on optimal growth conditions for BC production and understanding of the fundamental biological processes are imperative. In this study, we report a novel analytical platform that can be used for studying the biology and optimizing growth conditions of cellulose producing bacteria. The platform is based on surface growth pattern of the organism and allows us to confirm that cellulose fibrils produced by the bacteria play a pivotal role towards their chemotaxis. The platform efficiently determines the impacts of different growth conditions on cellulose production and is translatable to static culture conditions. The analytical platform provides a means for fundamental biological studies of bacteria chemotaxis as well as systematic approach towards rational design and development of scalable bioprocessing strategies for industrial production of bacterial cellulose. |
format | Online Article Text |
id | pubmed-5893554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58935542018-04-12 A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production Basu, Anindya Vadanan, Sundaravadanam Vishnu Lim, Sierin Sci Rep Article Bacterial cellulose (BC) is a biocompatible material with versatile applications. However, its large-scale production is challenged by the limited biological knowledge of the bacteria. The advent of synthetic biology has lead the way to the development of BC producing microbes as a novel chassis. Hence, investigation on optimal growth conditions for BC production and understanding of the fundamental biological processes are imperative. In this study, we report a novel analytical platform that can be used for studying the biology and optimizing growth conditions of cellulose producing bacteria. The platform is based on surface growth pattern of the organism and allows us to confirm that cellulose fibrils produced by the bacteria play a pivotal role towards their chemotaxis. The platform efficiently determines the impacts of different growth conditions on cellulose production and is translatable to static culture conditions. The analytical platform provides a means for fundamental biological studies of bacteria chemotaxis as well as systematic approach towards rational design and development of scalable bioprocessing strategies for industrial production of bacterial cellulose. Nature Publishing Group UK 2018-04-10 /pmc/articles/PMC5893554/ /pubmed/29636541 http://dx.doi.org/10.1038/s41598-018-23701-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Basu, Anindya Vadanan, Sundaravadanam Vishnu Lim, Sierin A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production |
title | A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production |
title_full | A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production |
title_fullStr | A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production |
title_full_unstemmed | A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production |
title_short | A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production |
title_sort | novel platform for evaluating the environmental impacts on bacterial cellulose production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5893554/ https://www.ncbi.nlm.nih.gov/pubmed/29636541 http://dx.doi.org/10.1038/s41598-018-23701-y |
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